标题：Hepatitis B virus inhibits intrinsic RIG-I and RIG-G immune signaling via inducing miR146a
作者：Hou, Zhaohua; Zhang, Jian; Han, Qiuju; Su, Chenhe; Qu, Jing; Xu, Dongqing; Zhang, Cai; Tian, Zhigang
作者机构：[Hou, Zhaohua; Zhang, Jian; Han, Qiuju; Su, Chenhe; Qu, Jing; Xu, Dongqing; Zhang, Cai] Shandong Univ, Sch Pharmaceut Sci, Inst Immunopharmaceut Sci, 更多
通讯作者：Zhang, J;Tian, ZG;Tian, ZG;Tian, ZG;Tian, ZG
通讯作者地址：[Zhang, J]Shandong Univ, Sch Pharmaceut Sci, Inst Immunopharmaceut Sci, Jinan, Peoples R China;[Tian, ZG]Zhejiang Univ, Affiliated Hosp 1, Coll Med,St 更多
摘要：Previous studies showed that hepatitis B virus (HBV), as a latency invader, attenuated host anti-viral immune responses. miRNAs were shown to be involved in HBV infection and HBV-related diseases, however, the precise role of miRNAs in HBV-mediated immunosuppression remains unclear. Here, we observed that down-regulated RIG-I like receptors might be one critical mechanism of HBV-induced suppression of type I IFN transcription in both HBV+ hepatoma cell lines and liver cancer tissues. Then, miR146a was demonstrated to negatively regulate the expression of RIG-I-like receptors by directly targeting both RIG-I and RIG-G. Further investigation showed that antagonizing miR146a by anti-sense inhibitors or sponge approach accelerated HBV clearance and reduced HBV load both in vitro and in a HBV-carrying mouse model. Therefore, our findings indicated that HBV-induced miR146a attenuates cell-intrinsic anti-viral innate immunity through targeting RIG-I and RIG-G, and silencing miR146a might be an effective target to reverse HBV-induced immune suppression.