标题:Deregulation of microRNA expression occurs early and accumulates in early stages of HBV-associated multistep hepatocarcinogenesis
作者:Gao, Peng; Wong, Carmen Chak-Lui; Tung, Edmund Kwok-Kwan; Lee, Joyce Man-Fong; Wong, Chun-Ming; Ng, Irene Oi-Lin
作者机构:[Gao, Peng; Wong, Carmen Chak-Lui; Tung, Edmund Kwok-Kwan; Lee, Joyce Man-Fong; Wong, Chun-Ming; Ng, Irene Oi-Lin] Univ Hong Kong, Li Ka Shing Fac Med 更多
通讯作者:Wong, CM
通讯作者地址:[Wong, CM]Univ Hong Kong, Dept Pathol, Queen Mary Hosp, Univ Pathol Bldg, Hong Kong, Hong Kong, Peoples R China.
来源:JOURNAL OF HEPATOLOGY
出版年:2011
卷:54
期:6
页码:1177-1184
DOI:10.1016/j.jhep.2010.09.023
关键词:microRNA expression; HBV-associated multistep; Hepatocarcinogenesis
摘要:Background & Aims: Deregulation of microRNAs (miRNAs) plays an important role in human carcinogenesis. However, miRNA deregulation in the pre-malignant lesions and expression changes during multistep hepatocarcinogenesis remain elusive.; Methods: In this study, we investigated the expression changes of seven cancer-related miRNAs during the early stages of HBV related hepatocarcinogenesis. miRNA was extracted from formalin fixed paraffin embedded (FFPE) dysplastic nodules (DN), small HCCs, and their corresponding non-tumorous livers. Expression changes of miRNAs were examined by real-time RT-qPCR.; Results: We found that down-regulation of miR-145 and miR-199b and up-regulation of miR-224 were frequently observed in pre-malignant DNs and these changes persisted throughout HCC development. Restoration of miR-145 in both HepG2 and Hep3B HCC cells significantly inhibited cell proliferation and reduced cell migration and cell invasion. Furthermore, these inhibitory functions of miR-145 could be substantially reduced by an anti-miR-145 inhibitor.; Conclusions: Our results showed that miRNA deregulation was an early event and accumulated throughout the various steps of HBV-associated hepatocarcinogenesis. Our findings also suggest that miR-145 is a candidate tumor suppressive miRNA and may play an important role in HCC development. (C) 2010 European Association for the Study of the Liver. Published by Elsevier B.V. All rights reserved.
收录类别:SCOPUS;SCIE
WOS核心被引频次:88
Scopus被引频次:90
资源类型:期刊论文
原文链接:https://www.scopus.com/inward/record.uri?eid=2-s2.0-79956081820&doi=10.1016%2fj.jhep.2010.09.023&partnerID=40&md5=4c6e7ffb0ab7cd3e05be0b75426eb897
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