标题：Metabolic engineering for improving l-tryptophan production in Escherichia coli
作者：Niu, Hao; Li, Ruirui; Liang, Quanfeng; Qi, Qingsheng; Li, Qiang; Gu, Pengfei
作者机构：[Niu, Hao; Li, Ruirui; Li, Qiang; Gu, Pengfei] Univ Jinan, Sch Biol Sci & Technol, Jinan 250022, Shandong, Peoples R China.; [Liang, Quanfeng; Qi, Q 更多
通讯作者地址：[Gu, PF]Univ Jinan, Sch Biol Sci & Technol, Jinan 250022, Shandong, Peoples R China.
来源：JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY
关键词：Escherichia coli; Metabolic engineering; l-Tryptophan
摘要：l-Tryptophan is an important aromatic amino acid that is used widely in the food, chemical, and pharmaceutical industries. Compared with the traditional synthetic methods, production of l-tryptophan by microbes is environmentally friendly and has low production costs, and feed stocks are renewable. With the development of metabolic engineering, highly efficient production of l-tryptophan in Escherichia coli has been achieved by eliminating negative regulation factors, improving the intracellular level of precursors, engineering of transport systems and overexpression of rate-limiting enzymes. However, challenges remain for l-tryptophan biosynthesis to be cost-competitive. In this review, successful and applicable strategies derived from metabolic engineering for increasing l-tryptophan accumulation in E. coli are summarized. In addition, perspectives for further efficient production of l-tryptophan are discussed.