标题:Met-enkephalin inhibits ROS production through Wnt/β-catenin signaling in the ZF4 cells of zebrafish
作者:Liu Z.; Liu Y.; Gu Y.; Gao L.; Li A.; Liu D.; Kang C.; Pang Q.;等 更多
作者机构:[Liu, Z] Shandong Provincial Key Laboratory of Animal Cell and Developmental Biology, Anti-aging & Regenerative Medicine Research Institution, School 更多
通讯作者:Liu, D(liudongwu@sdut.edu.cn)
通讯作者地址:[Liu, D] School of Agricultural Engineering and Food Science, Shandong University of TechnologyChina;
来源:Fish and Shellfish Immunology
出版年:2019
卷:88
页码:432-440
DOI:10.1016/j.fsi.2019.03.016
关键词:Met-enkephalin; ROS; Wnt/β-catenin signaling; Zebrafish ZF4 cells
摘要:Opioid neuropeptides are developed early in the course of a long evolutionary process. As the endogenous messengers of immune system, opioid neuropeptides participate in regulating immune response. In this study, the mechanism that Met-enkephalin (M-ENK) inhibits ROS production through Wnt/β-catenin signaling was investigated in the ZF4 cells of zebrafish. ZF4 cells were exposed to 0, 10, 20, 40, 80, and 160 μM Met-enkephalin (M-ENK) for 24 h, and the cell viability was detected with 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyl tetrazolium bromide (MTT) assay. The cell viability was significantly increased by 10, 20, 40, 80, and 160 μM M-ENK. After ZF4 cells were exposed to 0, 20, 40, and 80 μM M-ENK for 24 h, the mRNA expression of Wnt10b, β-catenin, and CCAAT/enhancer binding protein α (C/EBPα) was significantly increased by 40 and 80 μM M-ENK. However, the mRNA and protein expression of GSK-3β was significantly decreased by 40 and 80 μM M-ENK. The protein expression of β-catenin was significantly induced by 40 and 80 μM M-ENK, while the protein expression of p-β-catenin was significantly decreased by 20, 40, and 80 μM M-ENK. In addition, the mRNA expression of CAT, SOD, and GSH-PX was significantly increased by 40 and 80 μM M-ENK. The levels of H 2 O 2 , ·OH, and O 2 ·- were significantly decreased, but the activity of CAT, SOD, and GSH-PX was significantly increased by 40 and 80 μM M-ENK. The fluorescence intensity of reactive oxygen species (ROS) was decreased, and that of mitochondrial membrane potential (MMP) was increased with the increase of M-ENK concentration in ZF4 cells. The results showed that M-ENK could induce Wnt/β-catenin signaling, which further inhibited ROS production through the induction of C/EBPα MMP, and the activities of antioxidant enzymes. © 2019 Elsevier Ltd
收录类别:SCOPUS
资源类型:期刊论文
原文链接:https://www.scopus.com/inward/record.uri?eid=2-s2.0-85062812034&doi=10.1016%2fj.fsi.2019.03.016&partnerID=40&md5=3e41546b34981f1bb06d56a4975261ac
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