标题:Preparation of Anti-Lomefloxacin Antibody and Development of an Indirect Competitive Enzyme-Linked Immunosorbent Assay for Detection of Lomefloxacin Residue in Milk
作者:Cao, Zhaozhen; Lu, Shengxin; Liu, Jinting; Zhan, Jinhua; Meng, Meng; Xi, Rimo
作者机构:[Meng, Meng; Xi, Rimo] Nankai Univ, Coll Pharm, Tianjin 300071, Peoples R China.; [Meng, Meng; Xi, Rimo] Nankai Univ, Tianjin Key Lab Mol Drug Res, 更多
通讯作者:Xi, RM
通讯作者地址:[Xi, RM]Nankai Univ, Coll Pharm, Tianjin 300071, Peoples R China.
来源:ANALYTICAL LETTERS
出版年:2011
卷:44
期:6
页码:1100-1113
DOI:10.1080/00032719.2010.511733
关键词:Antibody; Cross-reactivity; Drug residue; Immunoassay; Lomefloxacin
摘要:Lomefloxacin has been increasingly used in veterinary medicine to treat microbial infections. To avoid using a complicated instrumental method to detect lomefloxacin residue in food, a simple and convenient indirect competitive enzyme-linked immunosorbent assay method has been developed in this study. The antibody generated from immunogen of bovine serum albumin-lomefloxacin showed high sensitivity toward lomefloxacin with an IC50 value of 0.35ppb in PBS buffer and was suitable to be used as a screening assay to detect lomefloxacin residue in food products. The ELISA (Enzyme-Linked ImmunoSorbant Assay) developed in this study was compared with a commercial ELISA kit and significant improvement was achieved in terms of sensitivity and specificity. The antibody prepared showed excellent specificity with certain cross-reactivity with only two drugs including norfloxacin (17.5%) and fleroxacin (8.8%) among commonly used (fluoro)quinolones. The assay measured drug residue in defatted milk spiked with lomefloxacin with an inter-assay coefficient of variation 22.2% and an intra-assay coefficient of variation 18.2%. The average recovery rates at 0.1, 0.3, 0.6, 1.0, and 2.0 ppb were in the range of 120-85% for inter-assay and in the range of 113-90% for intra-assay.
收录类别:SCOPUS;SCIE
WOS核心被引频次:4
Scopus被引频次:4
资源类型:期刊论文
原文链接:https://www.scopus.com/inward/record.uri?eid=2-s2.0-79953723154&doi=10.1080%2f00032719.2010.511733&partnerID=40&md5=284a67ab42f2759a738c8a26c3438735
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