标题：Perifosine downregulates MDR1 gene expression and reverses multidrug-resistant phenotype by inhibiting PI3K/Akt/NF-kappa B signaling pathway in a human breast cancer cell line
作者：Lin, X.; Zhang, X.; Wang, Q.; Li, J.; Zhang, P.; Zhao, M.; Li, X.
作者机构：[Lin, X.; Wang, Q.; Li, J.; Zhao, M.] Shandong Univ, Dept Pathol, Prov Hosp, Jinan 250021, Peoples R China.; [Zhang, X.] Shandong Univ, Dept Pathol, 更多
通讯作者地址：[Wang, Q]Shandong Univ, Dept Pathol, Prov Hosp, Jinan 250021, Peoples R China.
关键词：perifosine; PI3K/Akt; breast cancer; multidrug resistant gene 1;; P-glycoprotein
摘要：P-glycoprotein (P-gp)-mediated multidrug resistance (MDR) is the major clinical impediment to chemotherapy of breast cancers. Down-regulation of PI3K/Akt pathway has been described as related to reversal of MDR in cancer cells. Here, we investigated the reversal effect on MDR phenotype of perifosine, a new Akt inhibitor, in breast cancer cell lines. In this study, MCF-7/ADM cells and MCF-7 cells were treated with different concentrations of perifosine. Our results suggested that perifosine reversed MDR partially by downregulation of P-gp expression and inhibition of PI3K/Akt/NF-kappa B pathway in the MCF-7/ADM cell line. The novel Akt inhibitor perifosine may be a promising new drug due to its ability to reverse MDR in human breast cancer cells.