标题：Resveratrol raises in vitro anticancer effects of paclitaxel in NSCLC cell line A549 through COX-2 expression
作者：Kong, Fanhua; Zhang, Runqi; Zhao, Xudong; Zheng, Guanlin; Wang, Zhou; Wang, Peng
作者机构：[Kong, Fanhua] Shandong Univ, Sch Med, Jinan 250000, Shandong, Peoples R China.; [Kong, Fanhua; Zhang, Runqi; Wang, Peng] Taian City Cent Hosp, Dept 更多
通讯作者：Wang, P;Wang, Z
通讯作者地址：[Wang, P]Taian City Cent Hosp, Dept Thorac Surg, Tai An 271000, Shandong, Peoples R China;[Wang, Z]Shandong Univ, Shandong Prov Hosp, Dept Thorac Surg 更多
来源：KOREAN JOURNAL OF PHYSIOLOGY & PHARMACOLOGY
关键词：A549; Cancer; Gene; Paclitaxel; Resveratrol
摘要：The aim of this study was to determine the raising anticancer effects of resveratrol (Res) on paclitaxel (PA) in non-small cell lung cancer (NSCLC) cell line A549.The 10 mu g/ml of Res had no effect on human fetal lung fibroblast MRC-5 cells or on A549 cancer cells and the 5 or 10 mu g/ml of PA also had no effect on MRC-5 normal cells. PA-L (5 g/ml) and PA-H (10 mu g/ml) had the growth inhibitory effects in NSCLC cell line A549, and Res increased these growth inhibitory effects. By flow cytometry experiment, after Res (5 mu g/mI)+PA-H (10 mu g/ml) treatment, the A549 cells showed the most apoptosic cells compared to other group treatments, and after additional treatment with Res, the apoptosic cells of both two PA concentrations were raised. Res+PA could reduce the mRNA and protein expressions of COX-2, and Res+PA could reduce the COX-2 related genes of VEGF, MMP-1, MMP-2, MMP-9, NF-kappa B, Bcl-2, BclxL, procollagen I, collagen I, collagen III and CTGF, TNF-alpha,IL-1(3, iNOS and raise the TIMP-1, TIMP-2, TIMP-3, IKB-alpha, p53, p21, caspase-3, caspase-8, caspase-9, Bax genes compared to the control cells and the PA treated cells. From these results, it can be suggested that Res could raise the anticancer effects of PA in A549 cells, thus Res might be used as a good sensitizing agent for PA.