标题:Global transcription engineering of brewer's yeast enhances the fermentation performance under high-gravity conditions
作者:Gao, Cuijuan; Wang, Zhikun; Liang, Quanfeng; Qi, Qingsheng
作者机构:[Qi, Qingsheng] Shandong Univ, Natl Glycoengn Res Ctr, Jinan 250100, Peoples R China.; [Gao, Cuijuan; Wang, Zhikun; Liang, Quanfeng; Qi, Qingsheng] 更多
通讯作者:Qi, Q
通讯作者地址:[Qi, QS]Shandong Univ, Natl Glycoengn Res Ctr, Jinan 250100, Peoples R China.
来源:APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
出版年:2010
卷:87
期:5
页码:1821-1827
DOI:10.1007/s00253-010-2648-6
关键词:Brewer's yeast; gTME; Ethanol; Metabolic engineering; High gravity; fermentation
摘要:Global transcription engineering was developed as a tool to reprogram gene transcription for eliciting new phenotypes important for technological applications (Science 2006, 314(5805):1565-1568). A recent report indicated that the beneficial growth advantage of yeast cells expressing the SPT15-300 mutation is the result of enhanced uptake and/or improved utilization of leucine and thus was seen only on defined media with low concentrations of leucine (Appl Environ Microbiol 2009, 75(19):6055-6061). Further investigation towards a leucine-prototrophic strain of industrial lager brewer's yeast indicated that integration one copy of SPT15-300 in SPT15 allele, however, did lead to an increased ethanol tolerance on complex rich medium at high gravity fermentation condition. Under brewing conditions, the SPT15-300 mutant produced 80.78 g/L ethanol from 200 g/L carbohydrates after 384 h, almost twice as much as that of the wild-type strain. The results convinced us that the effect of global regulator modification of yeast is at multi-genes level and is extremely complicated.
收录类别:EI;SCOPUS;SCIE
WOS核心被引频次:6
Scopus被引频次:8
资源类型:期刊论文
原文链接:https://www.scopus.com/inward/record.uri?eid=2-s2.0-77955552251&doi=10.1007%2fs00253-010-2648-6&partnerID=40&md5=0f165326560f695468befcc8be5cf55b
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