标题:Myosin Va mediates BDNF-induced postendocytic recycling of full-length TrkB and its translocation into dendritic spines
作者:Sui, Wen-Hai; Huang, Shu-Hong; Wang, Jue; Chen, Qun; Liu, Ting; Chen, Zhe-Yu
作者机构:[Sui, Wen-Hai; Huang, Shu-Hong; Chen, Qun; Liu, Ting; Chen, Zhe-Yu] Shandong Univ, Dept Neurobiol, Shandong Prov Key Lab Mental Disorders, CAS Ctr Exc 更多
通讯作者:Chen, ZY
通讯作者地址:[Chen, ZY]Shandong Univ, Dept Neurobiol, Shandong Prov Key Lab Mental Disorders, CAS Ctr Excellence Brain Sci,Sch Med, 44 Wenhua Xi Rd, Jinan 250012, 更多
来源:JOURNAL OF CELL SCIENCE
出版年:2015
卷:128
期:6
页码:1108-1122
DOI:10.1242/jcs.160259
关键词:BDNF; TrkB; NTRK2; Myosin Va; Myo5a; Recycling; Rab11
摘要:Brain-derived neurotrophic factor (BDNF) plays an important role in neuronal survival, neurite outgrowth and synaptic plasticity by activating the receptor tropomyosin receptor kinase B (TrkB, also known as NTRK2). TrkB has been shown to undergo recycling after BDNF stimulation. We have previously reported that full-length TrkB (TrkB-FL) are recycled through a Rab11-dependent pathway upon BDNF stimuli, which is important for the translocation of TrkB-FL into dendritic spines and for the maintenance of prolonged BDNF downstream signaling during long-term potentiation (LTP). However, the identity of the motor protein that mediates the local transfer of recycled TrkB-FL back to the plasma membrane remains unclear. Here, we report that the F-actin-based motor protein myosin Va (Myo5a) mediates the postendocytic recycling of TrkB-FL. Blocking the interaction between Rab11 and Myo5a by use of a TAT-tagged peptide consisting of amino acids 55-66 of the Myo5a ExonE domain weakened the association between TrkB-FL and Myo5a and thus impaired TrkB-FL recycling and BDNF-induced TrkB-FL translocation into dendritic spines. Finally, inhibiting Myo5a-mediated TrkB-FL recycling led to a significant reduction in prolonged BDNF downstream signaling. Taken together, these results show that Myo5a mediates BDNF-dependent TrkB-FL recycling and contributes to BDNF-induced TrkB spine translocation and prolonged downstream signaling.
收录类别:SCOPUS;SCIE
WOS核心被引频次:5
Scopus被引频次:6
资源类型:期刊论文
原文链接:https://www.scopus.com/inward/record.uri?eid=2-s2.0-84925436888&doi=10.1242%2fjcs.160259&partnerID=40&md5=e810c25f7380ccdb961bd61084387bce
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