标题:Huaier Extract Induces Autophagic Cell Death by Inhibiting the mTOR/S6K Pathway in Breast Cancer Cells
作者:Wang, Xiaolong; Qi, Wenwen; Li, Yaming; Zhang, Ning; Dong, Lun; Sun, Mingjuan; Cun, Jinjing; Zhang, Yan; Lv, Shangge; Yang, Qifeng
作者机构:[Wang, Xiaolong; Zhang, Ning; Dong, Lun; Sun, Mingjuan; Cun, Jinjing; Yang, Qifeng] Shandong Univ, Qilu Hosp, Dept Breast Surg, Jinan 250100, Shandong 更多
通讯作者:Yang, QF
通讯作者地址:[Yang, QF]Shandong Univ, Qilu Hosp, Dept Breast Surg, Jinan 250100, Shandong, Peoples R China.
来源:PLOS ONE
出版年:2015
卷:10
期:7
DOI:10.1371/journal.pone.0131771
摘要:Huaier extract is attracting increased attention due to its biological activities, including antitumor, anti-parasite and immunomodulatory effects. Here, we investigated the role of autophagy in Huaier-induced cytotoxicity in MDA-MB-231, MDA-MB-468 and MCF7 breast cancer cells. Huaier treatment inhibited cell viability in all three cell lines and induced various large membranous vacuoles in the cytoplasm. In addition, electron microscopy, MDC staining, accumulated expression of autophagy markers and flow cytometry revealed that Huaier extract triggered autophagy. Inhibition of autophagy attenuated Huaier-induced cell death. Furthermore, Huaier extract inhibited the mammalian target of the rapamycin (mTOR)/S6K pathway in breast cancer cells. After implanting MDA-MB-231 cells subcutaneously into the right flank of BALB/c nu/nu mice, Huaier extract induced autophagy and effectively inhibited xenograft tumor growth. This study is the first to show that Huaier-induced cytotoxicity is partially mediated through autophagic cell death in breast cancer cells through suppression of the mTOR/S6K pathway.
收录类别:SCOPUS;SCIE
WOS核心被引频次:9
Scopus被引频次:15
资源类型:期刊论文
原文链接:https://www.scopus.com/inward/record.uri?eid=2-s2.0-84940048559&doi=10.1371%2fjournal.pone.0131771&partnerID=40&md5=b23d62b191207770ba4009f77c7399f3
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