标题：The human papillomavirus type 58 E7 oncoprotein modulates cell cycle regulatory proteins and abrogates cell cycle checkpoints
作者：Zhang, Weifang; Li, Jing; Kanginakudru, Sriramana; Zhao, Weiming; Yu, Xiuping; Chen, Jason J.
作者机构：[Zhang, Weifang; Li, Jing; Zhao, Weiming; Yu, Xiuping] Shandong Univ, Sch Med, Dept Microbiol, Jinan 250100, Shandong, Peoples R China.; [Zhang, Wei 更多
通讯作者地址：[Yu, XP]Shandong Univ, Sch Med, Dept Microbiol, Jinan 250100, Shandong, Peoples R China.
关键词：HPV-58; E7; Checkpoint; Rb; p130; Cdk2; p53; p21
摘要：HPV type 58 (HPV-58) is the third most common HPV type in cervical cancer from Eastern Asia, yet little is known about how it promotes carcinogenesis. In this study, we demonstrate that HPV-58 E7 significantly promoted the proliferation and extended the lifespan of primary human keratinocytes (PHKs). HPV-58 E7 abrogated the G1 and the postmitotic checkpoints, although less efficiently than HPV-16 ET Consistent with these observations, HPV-58 E7 down-regulated the cellular tumor suppressor pRb to a lesser extent than HPV-16 ET Similar to HPV-16 E7 expressing PHKs, Cdk2 remained active in HPV-58 E7 expressing PHKs despite the presence of elevated levels of p53 and p21. interestingly, HPV-58 E7 down-regulated p130 more efficiently than HPV-16 ET Our study demonstrates a correlation between the ability of down-regulating pRb/p130 and abrogating cell cycle checkpoints by HPV-58 E7, which also correlates with the biological risks of cervical cancer progression associated with HPV-58 infection. (C) 2009 Elsevier Inc. All rights reserved.