标题:Attenuation of Cisplatin-Induced Neurotoxicity by Cyanidin, a Natural Inhibitor of ROS-Mediated Apoptosis in PC12 Cells
作者:Li, Da-wei; Sun, Jing-yi; Wang, Kun; Zhang, Shuai; Hou, Ya-jun; Yang, Ming-feng; Fu, Xiao-yan; Zhang, Zong-yong; Mao, Lei-lei; Yuan, 更多
作者机构:[Li, Da-wei; Zhu, Mei-jia] Shandong Univ, Qianfoshan Hosp, Dept Neurol, Jinan 250014, Shandong, Peoples R China.; [Li, Da-wei; Zhang, Shuai; Hou, Ya 更多
通讯作者:Fan, CD
通讯作者地址:[Fan, CD]Taishan Med Univ, Key Lab Cerebral Microcirculat Univ Shandong, Tai An 271000, Shandong, Peoples R China.
来源:CELLULAR AND MOLECULAR NEUROBIOLOGY
出版年:2015
卷:35
期:7
页码:995-1001
DOI:10.1007/s10571-015-0194-6
关键词:Cyanidin; Cisplatin; Neurotoxicity; Apoptosis; Reactive oxygen species
摘要:Cisplatin-based chemotherapy in clinic is severely limited by its adverse effect, including neurotoxicity. Oxidative damage contributes to cisplatin-induced neurotoxicity, but the mechanism remains unclearly. Cyanidin, a natural flavonoid compound, exhibits powerful antioxidant activity. Hence, we investigated the protective effects of cyanidin on PC12 cells against cisplatin-induced neurotoxicity and explored the underlying mechanisms. The results showed that cisplatin-induced cytotoxicity was completely reversed by cyanidin through inhibition of PC12 cell apoptosis, as proved by the attenuation of Sub-G1 peak, PARP cleavage, and caspases-3 activation. Mechanistically, cyanidin significantly inhibited reactive oxygen species (ROS)-induced DNA damage in cisplatin-treated PC12 cells. Our findings revealed that cyanidin as an apoptotic inhibitor effectively blocked cisplatin-induced neurotoxicity through inhibition of ROS-mediated DNA damage and apoptosis, predicating its therapeutic potential in prevention of chemotherapy-induced neurotoxicity.; Cisplatin caused DNA damage, activated p53, and subsequently induced PC12 cells apoptosis by triggering ROS overproduction. However, cyanidin administration effectively inhibited DNA damage, attenuated p53 phosphorylation, and eventually reversed cisplatin-induced PC12 cell apoptosis through inhibition ROS accumulation.
收录类别:SCOPUS;SCIE
WOS核心被引频次:8
Scopus被引频次:12
资源类型:期刊论文
原文链接:https://www.scopus.com/inward/record.uri?eid=2-s2.0-84942196127&doi=10.1007%2fs10571-015-0194-6&partnerID=40&md5=b29c1ab280775fc6370d162e25337e98
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