标题：Cell-Inspired All-Aqueous Microfluidics: From Intracellular Liquid–Liquid Phase Separation toward Advanced Biomaterials
作者：Ma Q.; Song Y.; Sun W.; Cao J.; Yuan H.; Wang X.; Sun Y.; Shum H.C.
作者机构：[Ma, Q] Department of Pharmaceutics, School of Pharmacy, Qingdao University, Qingdao, 266021, China;[ Song, Y] Wallace H Coulter Department of Biomedi 更多
通讯作者地址：[Ma, Q] Department of Pharmaceutics, School of Pharmacy, Qingdao UniversityChina;
关键词：advanced biomaterials; all-aqueous microfluidics; cell-inspiration; intracellular organelles; liquid–liquid phase separation
摘要：Living cells have evolved over billions of years to develop structural and functional complexity with numerous intracellular compartments that are formed due to liquid–liquid phase separation (LLPS). Discovery of the amazing and vital roles of cells in life has sparked tremendous efforts to investigate and replicate the intracellular LLPS. Among them, all-aqueous emulsions are a minimalistic liquid model that recapitulates the structural and functional features of membraneless organelles and protocells. Here, an emerging all-aqueous microfluidic technology derived from micrometer-scaled manipulation of LLPS is presented; the technology enables the state-of-art design of advanced biomaterials with exquisite structural proficiency and diversified biological functions. Moreover, a variety of emerging biomedical applications, including encapsulation and delivery of bioactive gradients, fabrication of artificial membraneless organelles, as well as printing and assembly of predesigned cell patterns and living tissues, are inspired by their cellular counterparts. Finally, the challenges and perspectives for further advancing the cell-inspired all-aqueous microfluidics toward a more powerful and versatile platform are discussed, particularly regarding new opportunities in multidisciplinary fundamental research and biomedical applications. © 2020 The Authors. Published by WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim