标题:Battery-triggered microfluidic paper-based multiplex electrochemiluminescence immunodevice based on potential-resolution strategy
作者:Wang, Shaowei; Ge, Lei; Zhang, Yan; Song, Xianrang; Li, Nianqiang; Ge, Shenguang; Yu, Jinghua
作者机构:[Wang, Shaowei; Ge, Lei; Zhang, Yan; Ge, Shenguang; Yu, Jinghua] Univ Jinan, Key Lab Chem Sensing & Anal, Univ Shandong, Sch Chem & Chem Engn, Jinan 2 更多
通讯作者:Yu, JH
通讯作者地址:[Yu, JH]Univ Jinan, Key Lab Chem Sensing & Anal, Univ Shandong, Sch Chem & Chem Engn, Jinan 250022, Peoples R China.
来源:LAB ON A CHIP
出版年:2012
卷:12
期:21
页码:4489-4498
DOI:10.1039/c2lc40707h
摘要:A potential-resolution strategy for multiplex electrochemiluminescence (ECL) immunoassay on a microfluidic paper-based analytical device (mu-PAD) was demonstrated for the first time, using tris( bipyridine)-ruthenium(II) (Ru(bpy)(3)(2+)) and carbon nanodots (CNDs) as the ECL labels for high-throughput ECL immunoassay on mu-PAD. Based on this strategy, simultaneous detection of four tumor markers using only two screen-printed carbon working electrodes (one electrode for two analytes) on an immunodevice consisting of a piece of patterned paper (denoted as mu-PECLI in this work) was realized, which is a simplification of the configuration of traditional mu-PADs. As a further development of mu-PECLI in low-cost and disposable applications, battery-triggered (constant-potential) ECL detection on mu-PECLI was developed, allowing the traditional electrochemical workstation to be abandoned. In addition, to exactly control the output voltage of the battery, a low-cost and simple voltage-controller was designed and fabricated for the first time. The battery-triggered ECL immunoassay principle on mu-PECLI is explained. We found that the simultaneous determination of two analytes in one paper working zone could be achieved by controlling the operational constant-potential (+1.2 V for Ru(bpy)(3)(2+) labels and -1.2 V for CNDs labels (vs. Ag/AgCl auxiliary electrode)) by simply reversing the connection mode. Finally, four tumor markers in human serum samples from the Tumor Hospital were assayed and the results obtained were in acceptable agreement with the reference values from parallel single-analyte test. This battery-triggered mu-PECLI provides a new strategy for high-throughput, low-cost, sensitive, automated and simultaneous multiplex immunoassay and point-of-care diagnosis.
收录类别:SCOPUS;SCIE
WOS核心被引频次:79
Scopus被引频次:79
资源类型:期刊论文
原文链接:https://www.scopus.com/inward/record.uri?eid=2-s2.0-84867294058&doi=10.1039%2fc2lc40707h&partnerID=40&md5=e4e9d8a9d1c30bf94d0710831c6d36bc
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