标题：Human reconstructed skin in a mouse model
作者：Mi J.; Chen S.; Xu L.; Wen J.; Xu X.; Wu X.
作者机构：[Mi, J] Shandong Provincial Key Laboratory of Oral Tissue Regeneration and Laboratory for Tissue Engineering and Regeneration, School of Stomatology, 更多
通讯作者地址：[Wu, X] Shandong Provincial Key Laboratory of Oral Tissue Regeneration and Laboratory for Tissue Engineering and Regeneration, School of Stomatology, 更多
来源：Methods in Molecular Biology
关键词：Cell grafting; Human reconstructed skin; Mouse model; Skin dermal cells; Skin epidermal cells
摘要：Currently, no ideal in vivo skin model, to exactly mimic the native human skin, has been utilized for laboratory and clinical application. Here, we describe a method to in vivo reconstitute a human skin model, so-called hRSK, by using culture-expanded skin cells. We grafted a mixture of dissociated human epidermal and dermal cells onto an excision wound on the back of immunodeficient mouse to generate the hRSK, and the hRSK, containing epidermis, dermis, and subcutis and also appendages such as hair follicles, histologically mirrors in situ human skin. © Springer Science+Business Media, LLC, part of Springer Nature 2019.