标题：Resveratrol ameliorates mitochondrial dysfunction but increases the risk of hypoglycemia following hemorrhagic shock
作者：Wang, Hao; Guan, Yuxia; Widlund, Anne Lykkegaard; Becker, Lance B.; Baur, Joseph A.; Reilly, Patrick M.; Sims, Carrie A.
作者机构：[Wang, Hao] Shandong Univ, Dept Crit Care Med, Qilu Hosp, Jinan 250100, Peoples R China.; [Wang, Hao; Guan, Yuxia; Reilly, Patrick M.; Sims, Carrie 更多
会议名称：44th Annual Meeting of the Western-Trauma-Association
会议日期：MAR 02-07, 2014
来源：JOURNAL OF TRAUMA AND ACUTE CARE SURGERY
关键词：Resveratrol; hemorrhagic shock; mitochondria; blood glucose; rats
摘要：BACKGROUND: Hemorrhagic shock (HS) may contribute to organ failure, by profoundly altering mitochondria' function. Resveratrol (RSV), a naturally occurring polyphenol, has been shown to promote mitochondria' function and regulate glucose homeostasis in diabetes. We hypothesized that RSV during resuscitation would ameliorate HS-induced mitochondrial dysfunction and improve hyperglycemia following acute blood loss.; METHODS: With the use a decompensated HS model, male Long-Evans rats (n = 6 per group) were resuscitated with lactated Ringer's solution with or without RSV (30 mg/kg) and were killed before hemorrhage (sham), at severe shock, following resuscitation, and 18 hours after resuscitation. At each time point, the liver and kidney mitochondria were isolated to assess individual respiratory complexes (CI, CII, and CIV) and the production of reactive oxygen species (ROS). Blood samples were assayed for glucose, insulin, corticosterone, total glucagon-like peptide (GLP-1), glucagon, and serum cytokine levels. The Homeostatic Model Assessment Insulin Resistance index was used to quantify insulin resistance.; RESULTS: RSV supplementation following HS significantly improved mitochondrial function and decreased mitochondrial ROS production in both liver and kidney. RSV-treated animals had significantly lower blood glucose levels following resuscitation when compared with sham animals (116.0 +/- 20.2 mg/dL vs. 227.7 +/- 8.3 mg/dL, p < 0.05) or those resuscitated with lactated Ringer's solution (116.0 +/- 20.2 mg/dL vs. 359.0 +/- 79.5 mg/dL, p < 0.05). RSV supplementation was associated with significantly decreased plasma insulin levels (1.0 +/- 0.4 ng/mL vs. 6.5 +/- 3.7 ng/mL, p < 0.05), increased total GLP-1 levels (385.8 +/- 56.6 ng/mL vs. 187.3 +/- 11.1 ng/mL, p < 0.05), and a lower natural Log Homeostatic Model Assessment Insulin Resistance index (1.30 +/- 0.42 vs. 4.18 +/- 0.68, p < 0.05) but had minimal effect on plasma corticosterone, glucagon, or cytokine levels.; CONCLUSION: Resuscitation with RSV restores mitochondria' function and decreases insulin resistance but may be associated with increased hypoglycemia. The observed antiglycemic effects of RSV may be mediated by decreased mitochondrial ROS and increased GLP-1 secretion. (Copyright (C) 2014 by Lippincott Williams & Wilkins)