标题：Twist 1 regulates the expression of PPAR gamma during hormone-induced 3T3-L1 preadipocyte differentiation: a possible role in obesity and associated diseases
作者：Ma, Wanshan; Lu, Sumei; Sun, Tao; Wang, Xiangdong; Ma, Yongmei; Zhang, Xiaoli; Zhao, Ruxing; Wang, Yunshan
作者机构：[Ma, Wanshan; Lu, Sumei; Sun, Tao; Ma, Yongmei] Shandong Univ, Shandong Prov Qianfoshan Hosp, Dept Lab Med, Jinan 250014, Shandong, Peoples R China.; 更多
通讯作者地址：[Wang, YS]Shandong Univ, Jinan Ctr Hosp, Med Res & Lab Diagnost Ctr, Jinan 250013, Shandong, Peoples R China.
来源：LIPIDS IN HEALTH AND DISEASE
关键词：Twist 1; Obesity; PPAR gamma; Adipocyte differentiation; Adipokines
摘要：Background: Twist 1 is highly expressed in adipose tissue and has been associated with obesity and related disorders. However, the molecular function of Twist 1 in adipose tissue is unclear. Twist 1 has been implicated in cell lineage determination and differentiation. Therefore, we investigated both the role of Twist 1 in adipocyte precursor mobilization and the relationship of Twist 1 with other molecular determinants of adipocyte differentiation.; Methods: We examined Twist 1 mRNA and protein expression in subcutaneous adipose tissues from diet-induced obese C57/BL6 mice and Wistar rats and in obese patients undergoing liposuction or adipose transplant surgeries. Twist 1 expression was measured on days 0, 2, 4, 8, and 12 of 3T3-L1 differentiation in vitro. The role of Twist 1 in adipogenesis was explored using retroviral interference of Twist 1 expression. Adipokine secretion was evaluated using a RayBio (R) Biotin Label-based Adipokine Array.; Results: Twist 1 mRNA and protein levels were reduced in diet-induced obese mice and rats and in obese humans. Twist 1 was upregulated during 3T3-L1 preadipocyte differentiation in vitro, beginning from the fourth day of differentiation induction. Retroviral interference of Twist 1 expression did not significantly impair lipid formation; however, retroviral interference induced PPAR gamma mRNA and protein expression on day 4 of differentiation induction. Adipokine array analyses revealed increased secretion of CXCR4 (19.55-fold), VEGFR1 (92.13-fold), L-21 R (63.55-fold), and IL-12 R beta 1 (59.66-fold) and decreased secretion of VEGFR3 (0.01-fold), TSLP R (0.071-fold), MIP-1 gamma (0.069-fold), TNF RI/TNFRSF1A (0.09-fold), and MFG-E8 (0.06-fold).; Conclusions: Twist 1 is a regulator of adipocyte gene expression although it is not likely to regulate differentiation. We identified PPAR gamma as a potential target of Twist 1 and found variation in the secretion of multiple adipokines, which might indicate a prospective mechanism linking Twist 1 expression with obesity or associated diseases.