标题:Study on Absorption Mechanism and Tissue Distribution of Fucoidan
作者:Bai, Xu; Zhang, E.; Hu, Bo; Liang, Hao; Song, Shuliang; Ji, Aiguo
作者机构:[Bai, Xu; Zhang, E.; Hu, Bo; Liang, Hao; Song, Shuliang; Ji, Aiguo] Shandong Univ, Marine Coll, Weihai 264209, Peoples R China.; [Ji, Aiguo] Shandon 更多
通讯作者:Song, S(songshuliang@sdu.edu.cn)
通讯作者地址:Song, SL; Ji, AG (corresponding author), Shandong Univ, Marine Coll, Weihai 264209, Peoples R China.; Ji, AG (corresponding author), Shandong Univ, Sc 更多
来源:MOLECULES
出版年:2020
卷:25
期:5
DOI:10.3390/molecules25051087
关键词:fucoidan; fluorescent labeling; clathrin; absorption; tissue; distribution
摘要:Fucoidan exhibits several pharmacological activities and is characterized by high safety and the absence of toxic side effects. However, the absorption of fucoidan is not well-characterized. In the present study, fucoidan were labeled with fluorescein isothiocyanate (FITC) and their ability to traverse a monolayer of Caco-2 cells was examined. The apparent permeability coefficients (Papp x 10(-6)) of FITC-labeled fucoidan (FITC-fucoidan) were 26.23, 20.15, 17.93, 16.11 cm/sec, respectively, at the concentration of 10 mu g/mL at 0.5, 1, 1.5 and 2 h. The absorption of FITC-fucoidan was suppressed by inhibitors of clathrin-mediated endocytosis, chlorpromazine, NH4Cl, and Dynasore; the inhibition rates were 84.24%, 74.61%, and 63.94%, respectively. This finding suggested that clathrin-mediated endocytosis was involved in fucoidan transport. Finally, tissue distribution of FITC-fucoidan was studied in vivo after injection of 50 mg/kg body weight into the tail vein of mice. The results showed that FITC-fucoidan targeted kidney and liver, reaching concentrations of 1092.31 and 284.27 mu g/g respectively after 0.5 h. In summary, the present work identified the mechanism of absorption of fucoidan and documented its tissue distribution, providing a theoretical basis for the future development of fucoidan applications.
收录类别:SCOPUS;SCIE
WOS核心被引频次:3
Scopus被引频次:5
资源类型:期刊论文
原文链接:https://www.scopus.com/inward/record.uri?eid=2-s2.0-85081012535&doi=10.3390%2fmolecules25051087&partnerID=40&md5=36f35714f397295c44a4ccad4ea71863
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