标题：A simple fluorescent probe for sensing cysteine over homocysteine and glutathione based on PET
作者：Fan, Wenlong ;Huang, Ximing ;Shi, Xiaomin ;Wang, Zhuo ;Lu, Zhengliang ;Fan, Chunhua ;Bo, Qibing
作者机构：[Fan, Wenlong ;Huang, Ximing ;Shi, Xiaomin ;Lu, Zhengliang ;Fan, Chunhua ;Bo, Qibing ] Key Laboratory of Chemical Sensing & Analysis in Universities o 更多
来源：Spectrochimica Acta - Part A: Molecular and Biomolecular Spectroscopy
摘要：A big challenge is the discrimination of sulfhydryl-containing amino acids due to their structural similarity. We designed and synthesized a simple fluorescent probe 3 for specific detection of cysteine based on photo-induced electron transfer (PET). The acrylate and BODIPY moieties in probe 3 act as a reaction site and reporter group, respectively. So the synergistic effect of the substituent groups endows probe 3 very strong green fluorescence at 525 nm (λex= 500 nm). The cleavage reaction induced by cysteine leads to acrylate hydrolysis, and thereby triggers PET on, which effectively quench the fluorescence of 3. Probe 3 exhibited a rapid response towards cysteine over homocysteine and glutathione. Probe 3 is successfully applied for sensing and imaging cysteine in vitro or in vivo cells with low cytotoxicity.
© 2016 Elsevier B.V.