标题:Tim-4 Inhibits NO Generation by Murine Macrophages
作者:Xu, Li-yun; Qi, Jian-ni; Liu, Xiao; Ma, Hong-xin; Yuan, Wei; Zhao, Pei-qing; Liang, Xiao-hong; Xu, Yong; Wang, Hong-xing; Xu, Xiao-y 更多
作者机构:[Xu, Li-yun; Qi, Jian-ni; Liu, Xiao; Ma, Hong-xin; Zhao, Pei-qing; Liang, Xiao-hong; Xu, Yong; Wang, Hong-xing; Xu, Xiao-yan; Wang, Wei; Ma, Chun-hong 更多
通讯作者:Gao, LF
通讯作者地址:[Gao, LF]Shandong Univ, Key Lab Expt Teratol, Shandong Prov Key Lab Infect & Immunol, Dept Immunol,Minist Educ,Sch Med, 44 Wenhua Xi Rd, Jinan 250012, 更多
来源:PLoS One
出版年:2015
卷:10
期:4
DOI:10.1371/journal.pone.0124771
摘要:Objective; T cell immunoglobulin-and mucin-domain-containing molecule-4 (Tim-4) receives much attention as a potentially negative regulator of immune responses. However, its modulation on macrophages has not been fully elucidated so far. This study aimed to identify the role of Tim-4 in nitric oxide (NO) modulation.; Methods; Macrophages were stimulated with 100 ng/ml LPS or 100 U/ml IFN-gamma. RT-PCR was performed to detect TIM-4 mRNA expression. Tim-4 blocking antibody and NF-kappa B inhibitory ligand were involved in the study. NO levels were assayed by Griess reaction. Phosphorylation of NF-kappa B, Jak2 or Stat1 was verified by western blot.; Results; Tim-4 was up-regulated in murine macrophages after interferon-gamma (IFN-gamma) stimulation. Tim-4 over-expression decreased NO production and inducible nitric oxide synthase (iNOS) expression in lipopolysaccharide (LPS) or IFN-gamma-stimulated macrophages. Consistently, Tim-4 blockade promoted LPS or IFN-gamma-induced NO secretion and iNOS expression. Tim-4 over-expression decreased LPS-induced nuclear factor kappa B (NF-kappa B) p65 phosphorylation in macrophages, which was abrogated by NF-kappa B inhibitory ligand. On the contrary, Tim-4 blocking increased LPS-induced NF-kappa B signaling, which was also abrogated by NF-kappa B inhibition. In addition, Tim-4 blockade promoted Jak2 and Stat1 phosphorylation in IFN-gamma stimulated macrophages.; Conclusion; These results indicate that Tim-4 is involved in negative regulation of NO production in macrophages, suggesting the critical role of Tim-4 in immune related diseases.
收录类别:SCIE
WOS核心被引频次:3
资源类型:期刊论文
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