标题:N-acetylcysteine inhibits in vivo oxidation of native low-density lipoprotein
作者:Cui, Yuqi; Narasimhulu, Chandrakala A.; Liu, Lingjuan; Zhang, Qingbin; Liu, Patrick Z.; Li, Xin; Xiao, Yuan; Zhang, Jia; Hao, Hong; 更多
作者机构:[Cui, Yuqi; Zhang, Qingbin; Cui, Lianqun] Shandong Univ, Dept Cardiol, Shandong Prov Hosp, Jinan 250100, Shandong, Peoples R China.; [Cui, Yuqi; Liu 更多
通讯作者:Liu, ZG
通讯作者地址:[Liu, ZG]Ohio State Univ, Dept Internal Med, Dorothy M Davis Heart & Lung Res Inst, Div Cardiovasc Med,Wexner Med Ctr, Columbus, OH 43210 USA.
来源:SCIENTIFIC REPORTS
出版年:2015
卷:5
DOI:10.1038/srep16339
摘要:Low-density lipoprotein (LDL) is non-atherogenic, while oxidized LDL (ox-LDL) is critical to atherosclerosis. N-acetylcysteine (NAC) has anti-atherosclerotic effect with largely unknown mechanisms. The present study aimed to determine if NAC could attenuate in vivo LDL oxidation and inhibit atherosclerosis. A single dose of human native LDL was injected intravenously into male C57BL/6 mice with and without NAC treatment. Serum human ox-LDL was detected 30 min after injection, reached the peak in 3 hours, and became undetectable in 12 hours. NAC treatment significantly reduced serum ox-LDL level without detectable serum ox-LDL 6 hours after LDL injection. No difference in ox-LDL clearance was observed in NAC-treated animals. NAC treatment also significantly decreased serum ox-LDL level in patients with coronary artery diseases and hyperlipidemia without effect on LDL level. Intracellular and extracellular reactive oxidative species (ROS) production was significantly increased in the animals treated with native LDL, or ox-LDL and in hyperlipidemic LDL receptor knockout (LDLR-/-) mice that was effectively prevented with NAC treatment. NAC also significantly reduced atherosclerotic plaque formation in hyperlipidemic LDLR-/-mice. NAC attenuated in vivo oxidation of native LDL and ROS formation from ox-LDL associated with decreased atherosclerotic plaque formation in hyperlipidemia.
收录类别:SCOPUS;SCIE
WOS核心被引频次:2
Scopus被引频次:2
资源类型:期刊论文
原文链接:https://www.scopus.com/inward/record.uri?eid=2-s2.0-84946605796&doi=10.1038%2fsrep16339&partnerID=40&md5=13396ab59de6c73b242726669a93ba25
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