标题：The clinical significance and underlying correlation of pStat-3 and integrin alpha v beta 6 expression in gallbladder cancer
作者：Liu Enyu; Wang Na; Zhao Chuanzong; Wang Ben; Wu Xiaojuan; Wang Yan; Li Zequn; Hong Jianguo; Wang Jiayong; Liang Benjia; Peng Cheng 更多 作者机构：[Liu Enyu; Zhao Chuanzong; Wang Ben; Li Zequn; Hong Jianguo; Wang Jiayong; Liang Benjia; Peng Cheng; Zhu Min; Zhang Zongli] Qilu Hosp Shandong Univ, D 更多
通讯作者：Liu, EY;Zhu, M;Zongli, ZL
通讯作者地址：[Liu, EY; Zhu, M; Zongli, ZL]Qilu Hosp Shandong Univ, Dept Gen Surg, Jinan 250012, Shandong, Peoples R China.
关键词：gallbladder cancer; integrin beta 6; pStat-3; immunohistochemistry;; tumorigenesis
摘要：Background: Both phosphorylated signal transducer and activator of transcription 3(pStat-3) and integrin alpha v beta 6 can play vital role in the development and progression of cancer. However, little is known about their expression correlation and clinical significance in gallbladder cancer(GBC).; Objective: The aim of our present study was to investigate the expression of pStat-3 and integrin alpha v beta 6, two proteins' correlation and their clinical significance in GBC tissues.; Results: The expression of pStat-3 and integrin alpha v beta 6 were both significantly associated with T stage, lymph node metastasis status, TNM stage (P = 0.008, P = 0.000, P = 0.000 and P = 0.036, P = 0.001, P = 0.000, respectively). IHC and Western blot showed their expressions in GBC tissues were higher than that in paraneoplastic tissues. Moderate positive correlation existed between the two proteins (r = 0.349, P < 0.001). The survival analysis by Kaplan-Meier and Cox regression model showed that GBC patients with pStat-3 or integrin alpha v beta 6 positive expression had a significantly poorer 2-year survival rate (P = 0.002 and 0.000, the log-rank test, respectively), and either marker could act as unfavorable independent prognostic factors(RR = 1.907, P = 0.021 and RR = 2.046, P = 0.038).; Materials and Methods: The expression levels of pStat-3 and integrin alpha v beta 6 were analyzed in GBC cancerous and paraneoplastic tissues of 97 cases via immunohistochemistry(IHC) and further validated by western blot method. Besides, SPSS software was used to observe their clinical significance as well as the two proteins' correlation.; Conclusion: pStat-3 and integrin alpha v beta 6 were indicators of tumor's progression and poor prognosis of patients with GBC. And the further study involving them may provide a helpful therapeutic target in prevention and treatment of GBC patients.