标题:Knockdown of Glutamate Cysteine Ligase Catalytic Subunit by siRNA Causes the Gold Nanoparticles-Induced Cytotoxicity in Lung Cancer Cells
作者:Liu, Min; Zhao, Yunxue; Zhang, Xiumei
作者机构:[Liu, Min; Zhao, Yunxue; Zhang, Xiumei] Shandong Univ, Dept Pharmacol, Sch Med, Jinan 250100, Shandong, Peoples R China.
通讯作者:Zhang, XM
通讯作者地址:[Zhang, XM]Shandong Univ, Dept Pharmacol, Sch Med, Jinan 250100, Shandong, Peoples R China.
来源:PLOS ONE
出版年:2015
卷:10
期:3
DOI:10.1371/journal.pone.0118870
摘要:Gold nanoparticles (GNPs) have shown promising medical applications in cancer treatment involved in the regulation of intracellular redox balance. Previously, we have reported that GNPs can trigger apoptosis and necrosis in human lung cancer cells (A549) when L-buthionine-sulfoximine (BSO) was used to decrease the expression of intracellular glutathione (GSH). Herein, we investigated the cytotoxicity of GNPs toward lung cancer cells under the glutamate cysteine ligase catalytic subunit (GCLC) was silenced by siRNA. Our results showed that GNPs cause apoptosis and necrosis in cells transfected with GCLC siRNA by elevating intracellular reactive oxygen species (ROS). These findings demonstrated that the regulation of glutathione synthesis by GCLC siRNA in A549 cells can initiate the gold nanoparticles-induced cytotoxicity.
收录类别:SCOPUS;SCIE
WOS核心被引频次:5
Scopus被引频次:6
资源类型:期刊论文
原文链接:https://www.scopus.com/inward/record.uri?eid=2-s2.0-84926026344&doi=10.1371%2fjournal.pone.0118870&partnerID=40&md5=992c98d60b3247a70f6e333f363a277f
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