标题：YdiV regulates Escherichia coli ferric uptake by manipulating the DNA-binding ability of Fur in a SlyD-dependent manner
作者：Zhang F.; Li B.; Dong H.; Chen M.; Yao S.; Li J.; Zhang H.; Liu X.;等 更多 作者机构：[Zhang, F] State Key Laboratory of Microbial Technology, Shandong University, 72 Binhai Road, Qingdao, 266237, China;[ Li, B] Key Laboratory of Rare a 更多
来源：Nucleic acids research
摘要：Iron is essential for all bacteria. In most bacteria, intracellular iron homeostasis is tightly regulated by the ferric uptake regulator Fur. However, how Fur activates the iron-uptake system during iron deficiency is not fully elucidated. In this study, we found that YdiV, the flagella gene inhibitor, is involved in iron homeostasis in Escherichia coli. Iron deficiency triggers overexpression of YdiV. High levels of YdiV then transforms Fur into a novel form which does not bind DNA in a peptidyl-prolyl cis-trans isomerase SlyD dependent manner. Thus, the cooperation of YdiV, SlyD and Fur activates the gene expression of iron-uptake systems under conditions of iron deficiency. Bacterial invasion assays also demonstrated that both ydiV and slyD are necessary for the survival and growth of uropathogenic E. coli in bladder epithelial cells. This reveals a mechanism where YdiV not only represses flagella expression to make E. coli invisible to the host immune system, but it also promotes iron acquisition to help E. coli overcome host nutritional immunity. © The Author(s) 2020. Published by Oxford University Press on behalf of Nucleic Acids Research.