标题：PlncRNA-1 induces apoptosis through the Her-2 pathway in prostate cancer cells
作者：Yang, Qing; Cui, Zi-Lian; Wang, Qin; Jin, Xun-Bo; Zhao, Yong; Wang, Mu-Wen; Song, Wei; Qu, Hua-Wei; Kang, Wei-Ting
作者机构：[Yang Qing] Department of Breast and Thyroid Surgery, Shandong Provincial Hospital Affiliated to Shandong University, Jinan, Shandong China.;[Cui Zili 更多 通讯作者地址：[Cui, ZL]Shandong Univ, Sch Med, Jinan, Shandong, Peoples R China;[Cui, ZL]Shandong Univ, Shandong Prov Hosp, Minimally Invas Urol Ctr, Jinan, Shandon 更多
关键词：apoptosis; cell cycle; Her-2; LNCaP; long noncoding RNA; PlncRNA-1;; prostate cancer
摘要：To determine whether PlncRNA-1 induces apoptosis in prostate cancer cells through the Her-2 pathway. The expression of PlncRNA-1, Her-2, and related cyclin proteins in 23 cases of prostate cancer and adjacent normal tissues was analyzed and compared. LNCaP cells were divided into a control group and an LNCaP-PlncRNA-1-siRNA experimental group. Normal prostate RWPE-1 cells were divided into an RWPE-1 control group and an RWPE-1-PlncRNA-1 experimental group. After PlncRNA-1 silencing and overexpression, changes in Her-2 and cyclinD1 expression levels were detected both in vivo and in vitro. In prostate cancer tissues, Her-2 and PlncRNA-1 were highly expressed and significantly correlated. In LNCaP cells, the expression of Her-2 and cyclinD1 decreased following the downregulation of PlncRNA-1 as assessed by real-time PCR and Western blotting. In RWPE-1 cells, the expression of Her-2 and cyclinD1 increased following PlncRNA-1 overexpression. Flow cytometry revealed that the proportion of LNCaP cells in G2/M phase was significantly increased after PlncRNA-1 silencing and that the proportion of RWPE-1 cells in G2/M phase was significantly decreased after PlncRNA-1 overexpression. Furthermore, animal experiments validated these results. In conclusion, in prostate cancer, PlncRNA-1 regulates the cell cycle and cyclinD1 levels and can also regulate proliferation and apoptosis in prostate cancer cells through the Her-2 pathway.