标题：An integration-free iPSC line (SDQLCHi012-A) derived from a patient with inflammatory bowel disease-28 carrying compound heterozygote mutations in IL10RA gene
作者：Ma, Yanyan; Zhang, Haiyan; Zhang, Shisong; Dong, Rui; Yang, Xiaomeng; Li, Yue; Guan, Jingyun; Gai Zhongtao; Yi, Liu
作者机构：[Ma, Yanyan; Zhang, Haiyan; Dong, Rui; Yang, Xiaomeng; Li, Yue; Guan, Jingyun; Gai Zhongtao; Yi, Liu] Shandong Univ, Qilu Childrens Hosp, Pediat Res I 更多
通讯作者：Gai, ZT;Yi, L
通讯作者地址：[Gai, ZT; Yi, L]Shandong Univ, Qilu Childrens Hosp, Pediat Res Inst, Jinan 250022, Shandong, Peoples R China.
来源：STEM CELL RESEARCH
摘要：Induced pluripotent stem cell (iPSC) line (SDQLCHi012-A) was generated from peripheral blood mononuclear cells of an 11-month-old male who was diagnosed as inflammatory bowel disease-28 caused by compound heterozygote for IL10RA mutations (c.188 + 1G > A and c.301C > T). Non-integrating episomal vectors coding OCT4, SOX2, KLF4, BCL-XL and MYC were used for reprogramming. The established iPSC line contained the same mutations identified in the patient, showed a normal karyotype, differentiation potential in vitro and expressed pluripotency markers.