标题:Improving cellulase productivity of Penicillium oxalicum RE-10 by repeated fed-batch fermentation strategy
作者:Han, Xiaolong; Song, Wenxia; Liu, Guodong; Li, Zhonghai; Yang, Piao; Qu, Yinbo
作者机构:[Han, Xiaolong; Liu, Guodong; Yang, Piao; Qu, Yinbo] Shandong Univ, State Key Lab Microbial Technol, Jinan 250100, Peoples R China.; [Song, Wenxia] 更多
通讯作者:Qu, Yinbo
通讯作者地址:[Qu, YB]Shandong Univ, State Key Lab Microbial Technol, Jinan 250100, Peoples R China.
来源:BIORESOURCE TECHNOLOGY
出版年:2017
卷:227
页码:155-163
DOI:10.1016/j.biortech.2016.11.079
关键词:Penicillium oxalicum; Cellulase; Response surface methodology; Repeated; fed-batch fermentation
摘要:Medium optimization and repeated fed-batch fermentation were performed to improve the cellulase productivity by P. oxalicum RE-10 in submerged fermentation. First, Plackett-Burman design (PBD) and central composite design (CCD) were used to optimize the medium for cellulase production. PBD demonstrated wheat bran and NaNO3 had significant influences on cellulase production. The CCD results showed the maximum filter paper activity (FPA) production of 8.61 U/mL could be achieved in Erlenmeyer flasks. The maximal FPA reached 12.69 U/mL by submerged batch fermentation in a 7.5-L stirred tank, 1.76-fold higher than that on the original medium. Then, the repeated fed-batch fermentation strategy was performed successfully for increasing the cellulase productivity from 105.75 U/L/h in batch fermentation to 158.38 U/L/h. The cellulase activity and the glucan conversion of delignined corn cob residue hydrolysis had no significant difference between the enzymes sampled from different cycles of the repeated fed-batch fermentation and that from batch culture. (C) 2016 Elsevier Ltd. All rights reserved.
收录类别:EI;SCOPUS;SCIE
WOS核心被引频次:8
Scopus被引频次:9
资源类型:期刊论文
原文链接:https://www.scopus.com/inward/record.uri?eid=2-s2.0-85006976808&doi=10.1016%2fj.biortech.2016.11.079&partnerID=40&md5=0ab30886be678387409889723be50f7e
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