标题：The mechanism through which octreotide inhibits hepatic stellate cell activity
作者机构：[Wang, J] Clinical Test Department of Shandong Qianfoshan Hospital, Jinan, Shandong 250014, China;[ Wang, L] Department of Pathology, Shandong Univers 更多
通讯作者地址：[Han, B]Shandong Univ, Sch Med, Dept Pathol, 44 Wenhua Xi Rd, Jinan 250012, Shandong, Peoples R China.
来源：Molecular medicine reports
关键词：Fibrosis;Hepatic stellate cell;Transcription factor;Vascular endothelial growth factor
摘要：Hepatic stellate cells (HSCs) are important in the development of liver fibrosis and in the pathogenesis of portal hypertension. Octreotide, an analogue of somatostatin, has been demonstrated to effectively treat fibrosis and portal hypertension; however, its relative mechanism in HSCs remains unknown. LX-2, the immortalized HSC line, was used to study the mechanism whereby octreotide functions at different concentrations. Real-time polymerase chain reaction (PCR) and western blot analysis were used to analyze the expression of fibrosis markers and transcription factors following treatment with octreotide. Soluble secreted endothelin-1 (ET-1), collagen I and vascular endothelial growth factor (VEGF) were assessed in the supernatants of cultured cells by enzyme-linked immunosorbent assay (ELISA). In the present study, it was shown that octreotide was able to inhibit the proliferative ability of the LX-2 cells and decrease the expression of transforming growth factor β (TGF-β), α-smooth muscle actin (α -SMA) and smad-4a. The transcription factors, including c-Jun and sp-1, were downregulated in a dose-dependent manner following treatment with octreotide. The levels of ET-1 and collagen I in the supernatant decreased significantly in contrast with the normal levels, whereas the levels of VEGF in the LX-2 cells and the supernatant increased at a high octreotide concentration (10-5 nM). Octreotide may exert its effects on ET-1 or other targeting genes in HSCs through the downregulation of c-Jun and specificity protein 1 (sp-1), and the increased levels of VEGF may be the reason for the side effects observed at high concentrations of octreotide.