标题：Inhibitory receptor immunoglobulin-like transcript 4 was highly expressed in primary ductal and lobular breast cancer and significantly correlated with IL-10
作者：Liu, Jie; Wang, Linlin; Gao, Wei; Li, Liwen; Cui, Xia; Yang, Hongyan; Lin, Wenli; Dang, Qi; Zhang, Nan; Sun, Yuping
作者机构：[Liu, Jie; Wang, Linlin; Gao, Wei; Cui, Xia; Yang, Hongyan; Lin, Wenli; Dang, Qi; Zhang, Nan; Sun, Yuping] Shandong Univ, Jinan Cent Hosp, Dept Oncol, 更多
通讯作者地址：[Sun, YP]Shandong Univ, Jinan Cent Hosp, Dept Oncol, 105 Jie Fang Rd, Jinan 250013, Shandong, Peoples R China.
关键词：Immunoglobulin-like transcript 4; Interleukin-10; Tumor infiltrating; lymphocytes; Immunohistochemistry; Breast cancer
摘要：Background: Immunoglobulin-like transcript 4 (ILT4) is an inhibitory molecule involved in immune response and has recently been identified to be strongly inducible by IL-10. The aim of the present study was to examine the associations of ILT4 expression with clinicopathological characteristics and IL-10 expression in primary ductal and lobular breast cancer.; Methods: We studied the expression of ILT4 in 4 cancer cell lines, 117 primary tumor tissues and 97 metastatic lymph nodes from patients with primary ductal and lobular breast cancer by reverse transcription-polymerase chain reaction, western blot or immunohistochemistry analysis. Additionally, IL-10 expression was also investigated using immunohistochemistry in primary tumor tissues. Then the relationship between ILT4 expression and clinicopathological characteristics/IL-10 expression was evaluated.; Results: ILT4 was highly expressed in all 4 human breast cancer cell lines on both mRNA and protein levels. In primary tumor tissues, ILT4 or IL-10 was expressed in the cell membrane, cytoplasm, or both; the positive rate of ILT4 and IL-10 expression was 60.7% (71/117) and 80.34% (94/117), respectively. ILT4 level was significantly correlated with IL-10 (r = 0.577; p < 0.01). Furthermore, the expression of ILT4 or IL-10 was associated with less number of Tumor Infiltrating Lymphocytes (TILs) (p = 0.004 and 0.018, respectively) and more lymph node metastasis (p = 0.046 and 0.035, respectively).; Conclusion: Our data demonstrated the association of ILT4 and IL-10 expression in human breast cancer, suggesting their important roles in immune dysfunction and lymph node metastases.