标题:CRL4B interacts with and coordinates the SIN3A-HDAC complex to repress CDKN1A and drive cell cycle progression
作者:Ji, Qinghong; Hu, Huili; Yang, Fan; Yuan, Jupeng; Yang, Yang; Jiang, Liangqian; Qian, Yanyan; Jiang, Baichun; Zou, Yongxin; Wang, Ya 更多
作者机构:[Ji, Qinghong; Hu, Huili; Yang, Fan; Yuan, Jupeng; Yang, Yang; Jiang, Liangqian; Qian, Yanyan; Jiang, Baichun; Zou, Yongxin; Wang, Yan; Shao, Changshu 更多
通讯作者:Gong, YQ
通讯作者地址:[Gong, YQ]Shandong Univ, Sch Med, Inst Mol Med & Genet, Key Lab Expt Teratol,Minist Educ, Jinan 250012, Peoples R China.
来源:JOURNAL OF CELL SCIENCE
出版年:2014
卷:127
期:21
页码:4679-4691
DOI:10.1242/jcs.154245
关键词:CRL4B; CDKN1A; SIN3A-HDAC; Cell cycle progression
摘要:CUL4B, a scaffold protein that assembles the CRL4B ubiquitin ligase complex, participates in the regulation of a broad spectrum of biological processes. Here, we demonstrate a crucial role of CUL4B in driving cell cycle progression. We show that loss of CUL4B results in a significant reduction in cell proliferation and causes G1 cell cycle arrest, accompanied by the upregulation of the cyclindependent kinase (CDK) inhibitors (CKIs) p21 and p57 (encoded by CDKN1A and CDKN1C, respectively). Strikingly, CUL4B was found to negatively regulate the function of p21 through transcriptional repression, but not through proteolysis. Furthermore, we demonstrate that CRL4B and SIN3A-HDAC complexes interact with each other and co-occupy the CDKN1A and CDKN1C promoters. Lack of CUL4B led to a decreased retention of SIN3-AHDAC components and increased levels of acetylated H3 and H4. Interestingly, the ubiquitylation function of CRL4B is not required for the stable retention of SIN3A-HDAC on the promoters of target genes. Thus, in addition to directly contributing to epigenetic silencing by catalyzing H2AK119 monoubiquitylation, CRL4B also facilitates the deacetylation function of SIN3A-HDAC. Our findings reveal a coordinated action between CRL4B and SIN3A-HDAC complexes in transcriptional repression.
收录类别:SCIE
WOS核心被引频次:16
资源类型:期刊论文
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