标题:Construction of neuron specific vector of human antisense noggin gene expression
作者:Zhou, Shengnian; Li, Chengshan; Wei, Xiansen; Liu, Liqing; Zhang, Zhengda
作者机构:[Zhou Shengnian] Department of Neurology, Qilu Hospital of Shandong University, Jinan, Shandong 250012, China.;[Li Chengshan] Department of Neurology, 更多
通讯作者地址:[Zhou, SN]Shandong Univ, Dept Neurol, Qilu Hosp, Jinan 250012, Shandong, Peoples R China.
来源:中国神经再生研究(英文版)
出版年:2010
卷:5
期:15
页码:1171-1174
DOI:10.3969/j.issn.1673-5374.2010.15.009
关键词:noggin;alphal-tubulin;expression vector;antisense nucleic acids;nerve cells;neural regeneration
摘要:The noggin gene is present in the central nervous system in embryonic and postnatal mammals,and plays an important role in maintaining nervous system development and physiological function.A 0.76-kb sequence of human noggin gene was cloned by polymerase chain reaction with the digestion site of Hind Ⅲ and Xba l on the 5\' end.The cloned fragment was reversely inserted into pCS2+[Tα1]-GFP plasmid,an neural cell-specific antisense eukaryotic expression vector.The plasmid expresses antisense for human noggin specifically in neurons,which may facilitate understanding of the physiological function of noggin.
收录类别:CSCD;SCIE
资源类型:期刊论文
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