标题:Tripartite Motif-Containing Protein 38 Negatively Regulates TLR3/4-and RIG-I-Mediated IFN-beta Production and Antiviral Response by Targeting NAP1
作者:Zhao, Wei; Wang, Lijuan; Zhang, Meng; Wang, Peng; Yuan, Chao; Qi, Jianni; Meng, Hong; Gao, Chengjiang
作者机构:[Zhao, Wei; Wang, Lijuan; Zhang, Meng; Wang, Peng; Yuan, Chao; Qi, Jianni; Gao, Chengjiang] Shandong Univ, Sch Med, Dept Immunol, Key Lab Expt Teratol 更多
通讯作者:Gao, CJ
通讯作者地址:[Gao, CJ]Shandong Univ, Sch Med, Dept Immunol, Key Lab Expt Teratol,Minist Educ, Jinan 250012, Shandong, Peoples R China.
来源:JOURNAL OF IMMUNOLOGY
出版年:2012
卷:188
期:11
页码:5311-5318
DOI:10.4049/jimmunol.1103506
摘要:Recognition of RNA virus through TLR and RIG-I-like receptor results in rapid expression of type I IFNs, which play an essential role in host antiviral responses. However, the mechanisms to terminate the production of type I IFNs are not well defined. In the current study, we identified a member of the tripartite motif (TRIM) family, TRIM38, as a negative regulator in TLR3/4- and RIG-I mediated IFN-beta signaling. Knockdown of TRIM38 expression by small interfering RNA resulted in augmented activation of IFN regulatory factor 3 and enhanced expression of IFN-beta, whereas overexpression of TRIM38 had opposite effects. Coimmunoprecipitation and colocalization experiments demonstrated that TRIM38 interacted with NF-kappa B activating kinase-associated protein 1 (NAP1), which is required for TLR-induced IFN regulatory factor 3 activation and IFN-beta production. As an E3 ligase, TRIM38 promoted K48-linked polyubiquitination and proteasomal degradation of NAP1. Thus, knockdown of TRIM38 expression resulted in higher protein level of NAP1 in primary macrophages. Consistent with the inhibitory roles in TLR3/4- and RIG-I-mediated IFN-beta signaling, knockdown of TRIM38 significantly inhibited the replication of vesicular stomatitis virus. Overexpression of TRIM38 resulted in enhanced replication of vesicular stomatitis virus. Therefore, our results demonstrate that TRIM38 is a negative regulator for TLR and RIG-I-mediated IFN-beta production by targeting NAP1 for ubiquitination and subsequent proteasome-mediated degradation. The Journal of Immunology, 2012, 188: 5311-5318.
收录类别:SCOPUS;SCIE
WOS核心被引频次:39
Scopus被引频次:41
资源类型:期刊论文
原文链接:https://www.scopus.com/inward/record.uri?eid=2-s2.0-84862102052&doi=10.4049%2fjimmunol.1103506&partnerID=40&md5=c0298c302f79fb7e6b015ff99d6b79ff
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