标题:Effect of non-cell corynebacterium parvum on differentiation and maturation of bone marrow-derived dendritic cells
作者:Liu,C.;Gao,S.;Qu,Z.;Guo,C.;Wu,P.;Shi,Y.;Zhang,L.
作者机构:[Liu, C] Department of Immunology, Shandong University School of Medicine, Jinan, China, Shandong Quality Inspection Center for Medical Devices, Shand 更多
通讯作者:Zhang, LN
通讯作者地址:[Zhang, LN]Shandong Univ, Sch Med, Dept Immunol, Jinan 250100, Peoples R China.
来源:Immunological Investigations: A Journal of Molecular and Cellular Immunology
出版年:2012
卷:41
期:8
页码:820-830
DOI:10.3109/08820139.2012.690165
关键词:Antigen presentation;CD8T cells;Corynebacterium parvum;Dendritic cells;NCPP
摘要:Corynebacterium parvum (CP), with their potent anti-tumor activities, has been well documented. Non-cell Corynebacterium Parvum (NCPP) is a neotype of biological preparation, which based on manipulating CP with nanotechnology. The present study was designed to investigate the effect of NCPP/CP on bone marrow derived dendritic cells (BMDCs) in tumor-bearing mice, especially focused on the differentiation and maturation of these BMDCs. BM cells from tumor-bearing mice administrated with NCPP/CP were analyzed by flow cytometry, which exhibit enhanced numbers of DCs and macrophages. In the meanwhile, flow cytometry analysis showed mild but significant difference for CD80 expression on these LPS- treated BMDCs between NCPP/CP administrated mice and the control animals. Furthermore, antigen presenting assay for these LPS-treated BMDCs showed significant difference for cytolytic assay of CD8T cells against B16 melanoma cells, which indicate that NCPP treatments have enhanced the cytolytic rates of CD8T cells from 47.9%±2.3% to 54.2%±2.4%. The data suggest that NCPP/CP treatment can efficiently facilitate the generation of BMDCs in vivo and enhance the maturation of these BMDCs in vitro.
收录类别:SCOPUS;SCIE
资源类型:期刊论文
原文链接:https://www.scopus.com/inward/record.uri?eid=2-s2.0-84870952197&doi=10.3109%2f08820139.2012.690165&partnerID=40&md5=7db90f8cbdc34fc4866ff500a5ce4b39
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