标题：Effect of non-cell corynebacterium parvum on differentiation and maturation of bone marrow-derived dendritic cells
作者机构：[Liu, C] Department of Immunology, Shandong University School of Medicine, Jinan, China, Shandong Quality Inspection Center for Medical Devices, Shand 更多
通讯作者地址：[Zhang, LN]Shandong Univ, Sch Med, Dept Immunol, Jinan 250100, Peoples R China.
来源：Immunological Investigations: A Journal of Molecular and Cellular Immunology
关键词：Antigen presentation;CD8T cells;Corynebacterium parvum;Dendritic cells;NCPP
摘要：Corynebacterium parvum (CP), with their potent anti-tumor activities, has been well documented. Non-cell Corynebacterium Parvum (NCPP) is a neotype of biological preparation, which based on manipulating CP with nanotechnology. The present study was designed to investigate the effect of NCPP/CP on bone marrow derived dendritic cells (BMDCs) in tumor-bearing mice, especially focused on the differentiation and maturation of these BMDCs. BM cells from tumor-bearing mice administrated with NCPP/CP were analyzed by flow cytometry, which exhibit enhanced numbers of DCs and macrophages. In the meanwhile, flow cytometry analysis showed mild but significant difference for CD80 expression on these LPS- treated BMDCs between NCPP/CP administrated mice and the control animals. Furthermore, antigen presenting assay for these LPS-treated BMDCs showed significant difference for cytolytic assay of CD8T cells against B16 melanoma cells, which indicate that NCPP treatments have enhanced the cytolytic rates of CD8T cells from 47.9%±2.3% to 54.2%±2.4%. The data suggest that NCPP/CP treatment can efficiently facilitate the generation of BMDCs in vivo and enhance the maturation of these BMDCs in vitro.