标题:A new group of anti-lipopolysaccharide factors from Marsupenaeus japonicus functions in antibacterial response
作者:Jiang, Hai-Shan;Zhang, Qing;Zhao, Yan-Ran;Jia, Wen-Ming;Zhao, Xiao-Fan;Wang, Jin-Xing
作者机构:[Jiang, H.-S] MOE Key Laboratory of Plant Cell Engineering and Germplasm Innovation, Shandong Provincial Key Laboratory of Animal Cells and Developmen 更多
通讯作者:Wang, JX
通讯作者地址:[Wang, JX]Shandong Univ, Sch Life Sci, Jinan 250100, Shandong, Peoples R China.
来源:Developmental and Comparative Immunology: Ontogeny, Phylogeny, Aging: The Official Journal of the International Society of Developmental and Comparative Immunology
出版年:2015
卷:48
期:1
页码:33-42
DOI:10.1016/j.dci.2014.09.001
关键词:Anti-lipopolysaccharide factors;Antimicrobial peptide;Bacterial clearance;RNA interference;Shrimp innate immunity;Marsupenaeus japonicus
摘要:Anti-lipopolysaccharide factors (ALFs) are a group of critical effector molecules with a broad spectrum of antimicrobial activities in crustaceans. Four groups of ALFs (A, B, C, and D) have been identified in peneaid shrimp. In the study, we identified a new group of ALFs (designated as MjALF-E) from Marsupenaeus japonicus. This new group (group E) included MjALF-E1 and E2. MjALF-E1 was highly expressed in hemocytes, heart, and intestine, whereas E2 was highly expressed in gills, stomach, and intestine. Expressions of both MjALF-E1 and E2 were upregulated by bacterial challenge. Synthesized LPS-binding domain peptides of MjALF-E1 and E2 strongly bind to bacterial cell wall components lipopolysaccharide (LPS) and peptidoglycan (PGN). The recombinant rMjALF-E2 showed relatively weak binding activity to LPS and PGN. Both synthesized peptides and rMjALF-E2 exhibited antimicrobial activity against Gram-negative bacteria, whereas rMjALF-E2 could promote the clearance of bacteria in vivo. After knockdown of MjALF-E2 and infection with Vibrio anguillarum, shrimp showed high and rapid mortality compared with GFPi shrimp. These results suggest that MjALF-Es serves a protective function against bacterial infection in shrimp. (C) 2014 Elsevier Ltd. All rights reserved.
收录类别:SCOPUS;SCIE
WOS核心被引频次:17
Scopus被引频次:19
资源类型:期刊论文
原文链接:https://www.scopus.com/inward/record.uri?eid=2-s2.0-84907812344&doi=10.1016%2fj.dci.2014.09.001&partnerID=40&md5=d1e8df96a27931d783d80a4b30a57fcf
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