标题：A new group of anti-lipopolysaccharide factors from Marsupenaeus japonicus functions in antibacterial response
作者：Jiang, Hai-Shan;Zhang, Qing;Zhao, Yan-Ran;Jia, Wen-Ming;Zhao, Xiao-Fan;Wang, Jin-Xing
作者机构：[Jiang, H.-S] MOE Key Laboratory of Plant Cell Engineering and Germplasm Innovation, Shandong Provincial Key Laboratory of Animal Cells and Developmen 更多
通讯作者地址：[Wang, JX]Shandong Univ, Sch Life Sci, Jinan 250100, Shandong, Peoples R China.
来源：Developmental and Comparative Immunology: Ontogeny, Phylogeny, Aging: The Official Journal of the International Society of Developmental and Comparative Immunology
关键词：Anti-lipopolysaccharide factors;Antimicrobial peptide;Bacterial clearance;RNA interference;Shrimp innate immunity;Marsupenaeus japonicus
摘要：Anti-lipopolysaccharide factors (ALFs) are a group of critical effector molecules with a broad spectrum of antimicrobial activities in crustaceans. Four groups of ALFs (A, B, C, and D) have been identified in peneaid shrimp. In the study, we identified a new group of ALFs (designated as MjALF-E) from Marsupenaeus japonicus. This new group (group E) included MjALF-E1 and E2. MjALF-E1 was highly expressed in hemocytes, heart, and intestine, whereas E2 was highly expressed in gills, stomach, and intestine. Expressions of both MjALF-E1 and E2 were upregulated by bacterial challenge. Synthesized LPS-binding domain peptides of MjALF-E1 and E2 strongly bind to bacterial cell wall components lipopolysaccharide (LPS) and peptidoglycan (PGN). The recombinant rMjALF-E2 showed relatively weak binding activity to LPS and PGN. Both synthesized peptides and rMjALF-E2 exhibited antimicrobial activity against Gram-negative bacteria, whereas rMjALF-E2 could promote the clearance of bacteria in vivo. After knockdown of MjALF-E2 and infection with Vibrio anguillarum, shrimp showed high and rapid mortality compared with GFPi shrimp. These results suggest that MjALF-Es serves a protective function against bacterial infection in shrimp. (C) 2014 Elsevier Ltd. All rights reserved.