标题：Enhanced plant antioxidant capacity and biodegradation of phenol by immobilizing peroxidase on amphoteric nitrogen-doped carbon dots
作者：Chen, Qiong; Man, Han; Zhu, Lin; Guo, Zhijiang; Wang, Xiuli; Tu, Jiangping; Jin, Gong; Lou, Jun; Zhang, Lin; Ci, Lijie
作者机构：[Chen, Qiong; Man, Han; Zhang, Lin; Ci, Lijie] Shandong Univ, Key Lab Liquid Solid Struct Evolut & Proc Mat, SDU & Rice Joint Ctr Carbon Nanomat, Mini 更多
通讯作者：Zhang, Lin;Zhang, L;Ci, LJ
通讯作者地址：[Zhang, L; Ci, LJ]Shandong Univ, Key Lab Liquid Solid Struct Evolut & Proc Mat, SDU & Rice Joint Ctr Carbon Nanomat, Minist Educ,Sch Mat Sci & Engn, J 更多
关键词：Amphoteric nitrogen-doped carbon dots; Horseradish peroxidase; Enzyme; immobilization; Plant antioxidant capacity; Phenol biodegradation
摘要：A green method of immobilizing enzyme on biocompatible nitrogen-doped carbon dots (N-CDs) is proposed in this research. The N-CDs that were prepared in an environmentally friendly manner can improve plant antioxidant capacity to alleviate growth inhibition under stress. The fluorescence signal emitted by N-CDs present in vivo in plants indicates that N-CDs can immobilize peroxidase without a crosslinker. After immobilization, N-CDs-supported horseradish peroxidase (N-CDs@HRP) can significantly enhance peroxidase activity, stability, and phenol removal capacity when compared with free HRP. The N-CDs-immobilized enzyme with high activity and stability can be broadly applied to different environments and under harsh conditions.