标题：NCoR negatively regulates adipogenic differentiation of mesenchymal stem cells
作者：Gao Hong-Wei; Liu Lan; Xing De-Guo; Liu Zhong-Hao; Ren Peng; Li Zhi-Qiang; Shan Guo-Qiang; Gong Ming-Zhi
作者机构：[Gao Hong-Wei; Xing De-Guo; Liu Zhong-Hao; Ren Peng; Gong Ming-Zhi] Shandong Univ, Dept Trauma & Orthopaed, Hosp 2, Jinan 250033, Shandong, Peoples R 更多
通讯作者地址：[Gong, MZ]Shandong Univ, Dept Trauma & Orthopaed, Hosp 2, 247 Beiyuan St, Jinan 250033, Shandong, Peoples R China.
来源：IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-ANIMAL
关键词：NCoR; MSCs; Differentiation; siRNA; Over-expression; C/EBP alpha; PPAR; gamma
摘要：The nuclear receptor corepressor (NCoR) regulates the activities of gene transcription. Mesenchymal stem cells (MSCs) derived from bone marrow are multipotent cells which can differentiate into osteoblasts and adipocytes. This study was conducted to investigate the effects of NCoR on adipogenic differentiation of MSCs isolated from the rats. The results suggested that rat MSCs could differentiate into adipocytes successfully after cultured in adipogenic medium. NCoR protein determined by Western blot showed a lower expression in MSC-derived adipocytes, indicating that NCoR was involved in adipocyte differentiation of rat MSCs. It further proved that small interfering RNA (siRNA)-mediated knockdown of NCoR could promote cell viability and differentiation and enhance messenger RNA (mRNA) expression of lipoprotein lipase (LPL) and protein expression of CCAAT/enhancer binding protein-alpha (C/EBP alpha) and peroxisome proliferator-activated receptor-gamma (PPAR gamma). However, over-expression of NCoR exerted its functions in contrary to NCoR knockdown. It indicated that NCoR could negatively regulate adipogenic differentiation of rat MSCs.