标题：Determination of estrogens in water by HPLC-UV using cloud point extraction
作者：Wang L;Cai YQ;He B;Yuan CG;Shen DZ;Shao J;Jiang GB
作者机构：[Wang, L; Cai, YQ; He, B; Yuan, CG; Shen, DZ; Shao, J; Jiang, GB]Chinese Acad Sci, Ecoenvironm Sci Res Ctr, State Key Lab Environm Chem & 更多
会议名称：China/Japan/Korea Environmental Analytical Chemistry Symposium
会议日期：SEP 01-03, 2005
来源：Talanta: The International Journal of Pure and Applied Analytical Chemistry
关键词：estrogens;cloud point extraction;Triton X-114;non-ionic surfactant;HPLC-UV;POLYCYCLIC AROMATIC-HYDROCARBONS;ATOMIC-ABSORPTION-SPECTROMETRY;MEDIATED PHASE SEPARATIONS;ORGANIC-COMPOUNDS;CAPILLARY-ELECTROPHORESIS;LIQUID-CHROMATOGRAPHY;MASS SPECTROMETRY;TRACE AMOUNTS;PRECONCENTRATION;SEAWATER
摘要：A method based on cloud point extraction was developed to determine four kinds of estrogens: estriol (E3), estradiol (E2), estrone (El), and progesterone (P) in water by high performance liquid chromatography separation and ultraviolet detection (HPLC-UV). The non-ionic surfactant Triton X-114 was chosen as extractant solvent. The parameters affecting extraction efficiency, such as concentrations of Triton X-114 and Na2SO4, equilibration temperature, equilibration time and centrifugation time were evaluated and optimized. Under the optimum conditions, preconcentration factors of 99 for E3. 73 for E2. 152 for E I and 86 for P were obtained for 10 mL water sample. The detection of limitation was 0.23 ng mL(-1) for E3, 0.32 ng mL(-1) for E2, 0.25 ng mL(-1) for El and 5.0 ng mL-1 for P. The proposed method was successfully applied to the determination of trace amount of estrogens in wastewater treatment plant (WWTP) effluent water and exposure water with 10 ng mL-1 E2 for toxicological study in our lab. For the case of WWTP effluent water samples, no estrogen was found. The accuracy of the proposed method was tested by recovery measurements of spiked samples and good recoveries of 81.2-99.5% were obtained. (c) 2006 Published by Elsevier B.V.