标题:Short hairpin RNA-mediated MDR1 gene silencing increases apoptosis of human ovarian cancer cell line A2780/taxol
作者:Xu, Hui; Hong, Fan-zhen; Li, Su; Zhang, Ping; Zhu, Lin
作者机构:[Xu, H] Department of Obstetrics and Gynecology, Second Hospital of Shandong University, Jinan 250033, China;[ Hong, F.-Z] Department of Obstetrics an 更多
通讯作者:Zhu, L
通讯作者地址:[Zhu, L]Shandong Univ, Dept Obstet & Gynecol, Hosp 2, Jinan 250033, Peoples R China.
来源:中国癌症研究(英文版)
出版年:2012
卷:24
期:2
页码:138-142
DOI:10.1007/s11670-012-0138-3
关键词:Ovarian cancer;Short hairpin RNA;Paclitaxel
摘要:Objective:Recurrent ovarian cancer is often resistant to drugs such as paclitaxel.Short hairpin RNA (shRNA) targeting MDR1,a gene involved in the process of drug resistance,may be a promising strategy to overcome drug resistance.Methods:Construction and identification of eukaryotic expression plasmid of shRNA targeting on MDR1 gene.The plasmid was transiently transfected into human ovarian cancer cell line A2780/Taxol.Apoptosis was determined by flow cytometry using annexin V-FITC/PI double labeling.Expression of MDR1 mRNA was detected by quantitative polymerase chain reaction (qPCR) and P-glycoprotein expression was detected using Western blot.Results:The IC50 of paclitaxel in MDR1 shRNA-transfected group was significantly reduced (1.986±0.153) μmol/ml as compared with that in negative control (5.246±0.107)μmol/ml and empty vector-transfected group (5.212±0.075)μmol/ml (P<0.05).The percent of the relative reverse sensitivity to paclitaxel on A2780/Taxol cells was 67.1%,and the apoptotic rate was significantly increased [(6.977±0.333)%] compared with control [(1.637±0.111)%] and empty vector-transfected group [(1.663±0.114)%] (P<0.05).Expressions of MDR1 mRNA and P-glycoprotein were significantly reduced compared with control (P<0.05).Conclusion:The present study demonstrated that the eukaryotic expression plasmid of shRNA targeting on MDR1 inhibited the expression of MDR1 effectively,thus enhance the sensitivity of A2780/Taxol cells to paclitaxel.
收录类别:SCOPUS;SCIE
WOS核心被引频次:5
Scopus被引频次:7
资源类型:期刊论文
原文链接:https://www.scopus.com/inward/record.uri?eid=2-s2.0-84863687051&doi=10.1007%2fs11670-012-0138-3&partnerID=40&md5=88d47a76e3cfdaf48aaac08021491c18
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