标题:Sclerostin is differently immunolocalized in metaphyseal trabecules and cortical bones of mouse tibiae
作者:Hasegawa, T.;Amizuka, N.;Yamada, T.;Liu, Z.;Miyamoto, Y.;Sasaki, M.;Hongo, H.;Suzuki, R.;De Freitas, P.H.L.;Yamamoto, T.;Oda, K.
作者机构:[Hasegawa, T] Department of Developmental Biology of Hard Tissue, Graduate School of Dental Medicine, Hokkaido University, Sapporo, Japan;[ Amizuka, N 更多
通讯作者:Li, MQ
通讯作者地址:[Li, MQ]Shandong Univ, Sch Stomatol, Shandong Prov Key Lab Oral Biomed, Wenhua West Rd 44-1, Jinan 250012, Peoples R China.
来源:Biomedical Research
出版年:2013
卷:34
期:3
页码:153-159
DOI:10.2220/biomedres.34.153
关键词:Sclerostin;immunolocalized;trabecules
摘要:Sclerostin, an osteocyte-derived molecule, has been reported to serve as a negative regulator of osteoblastic activity as well as bone remodeling. However, there is no report that verified the regional difference for sclerostin synthesis, and in this study we have investigated immunolocalization of sclerostin by comparing dentin matrix protein (DMP) 1, an osteocyte-derived factor broadly expressed in tibial metaphyses and cortical bone. In metaphyseal primary trabecules, a site of bone modeling, strong DMP1-reactivity was observed in osteocytic lacunar-canalicular system (OLCS), while faint staining for sclerostin was visible only in a few osteocytes. In secondary trabecules, in which bone remodeling begins, some osteocytes showed intense sclerostin-immunopositivity, though there were many DMP1-positive osteocytes. In cortical bone, there were more osteocytes reactive for sclerostin, when compared with those in the secondary trabecules. Silver impregnation verified that immature, primary trabecules contained randomly-oriented OLCS, while mature, cortical bone showed geometrically well-arrangement of OLCS. Taken together, though DMP1 is broadly synthesized in bone, sclerostin appears to be abundantly synthesized in regular OLCS of cortical bone, but less produced in irregular OLCS as seen in primary trabecules, indicating the regional difference for sclerostin synthesis.
收录类别:SCOPUS;SCIE
WOS核心被引频次:7
Scopus被引频次:6
资源类型:期刊论文
原文链接:https://www.scopus.com/inward/record.uri?eid=2-s2.0-84879178317&doi=10.2220%2fbiomedres.34.153&partnerID=40&md5=3bf2a3c6f445556c74c3e957103dc7e6
TOP