标题：Influence of CXCR4/SDF-1 axis on E-cadherin/beta-catenin complex expression in HT29 colon cancer cells
作者：Wang, Lin; Li, Cui-Ling; Wang, Lei; Yu, Wen-Bin; Yin, Hai-Peng; Zhang, Guang-Yong; Zhang, Li-Feng; Li, Sheng; Hu, San-Yuan
作者机构：[Wang Lin] Department of General Surgery, Qilu Hospital, Shandong University, Jinan, Shandong 250012, China.;[Wang Lei] Department of General Surgery, 更多
通讯作者地址：[Hu, SY]Shandong Univ, Dept Gen Surg, Qilu Hosp, Jinan 250012, Shandong, Peoples R China.
来源：WORLD JOURNAL OF GASTROENTEROLOGY
关键词：CXCR4; Stromal cell-derived factor-1; E-cadherin; beta-catenin;; Phosphatidylinositol 3-kinase/AKT; Colon cancer
摘要：AIM: To study the influence of CXCR4/stromal cell-derived factor-1 (SDF-1) axis on E-cadherin/beta-catenin complex expression in HT29 colon cancer cells and its underlying mechanisms.; METHODS: Effect of SDF-1 on E-cadherin/beta-catenin expression was detected by immunocytochemistry. E-cadherin and p-catenin mRNA expression levels were measured by reverse transcriptase-polymerase chain reaction. SDF-1-induced phosphorylation of phosphatidylinositol 3-kinase (PI3K)/AKT and p-catenin was detected by Western blotting.; RESULTS: The E-cadherin and beta-catenin mRNA expression levels in HT29 cells were lower 48 h after incubated with SDF-1 at the concentrations of 20 and 40 ng/mL (P < 0.05). SDF-1-induced significant phosphorylation of PI3K/AKT and p-catenin. AMD3100 and LY294002 inhibited the phosphorylation cif PI3K/AKT and beta-catenin.; CONCLUSION: SDF-1 down-regulates the E-cadherin/beta-catenin complex expression in HT29 cells by decreasing mRNA synthesis and increasing p-catenin phosphorylation. (C) 2011 Baishideng. All rights reserved.