标题:Ultrasensitive chemiluminescence detection of DNA on a microfluidic paper-based analytical device
作者:Wang, Yanhu; Wang, Shoumei; Ge, Shenguang; Wang, Shaowei; Yan, Mei; Zang, Dejin; Yu, Jinghua
通讯作者:Yu, JH
作者机构:[Wang, Yanhu; Wang, Shoumei; Ge, Shenguang; Wang, Shaowei; Yan, Mei; Zang, Dejin; Yu, Jinghua] Univ Jinan, Sch Chem & Chem Engn, Key Lab Chem Sensing 更多
会议名称:2nd International Congress on Advanced Materials (ICAM)
会议日期:MAY 16-19, 2013
来源:MONATSHEFTE FUR CHEMIE
出版年:2014
卷:145
期:1
页码:129-135
DOI:10.1007/s00706-013-0971-1
关键词:Microfluidic paper-based chemiluminescence analytical device; DNA;; Chemiluminescence; Gold nanoparticles
摘要:A novel microfluidic paper-based chemiluminescence (CL) analytical device (mu PAD) with high-throughput, rapid, stable, reusable point-of-care (POC) testing and quantitative response for DNA was designed. Sodium periodate was used to modify mu PAD to covalently immobilize capture DNA. The paper device was fabricated by a low-cost, simple, and rapid wax screen-printing method. The developed paper-based CL immunodevice, combined with a typical luminol-H2O2 CL system, showed excellent analytical performance for the detection of DNA. Moreover, gold nanoparticles for signal DNA immobilization were used for signal-on CL assay of target DNA, which greatly improved the sensitivity. This study shows the successful integration of the mu PAD and the CL method to afford an easy-to-use, inexpensive, and portable alternative for POC monitoring.
收录类别:CPCI-S;SCIE
WOS核心被引频次:11
资源类型:会议论文
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