标题：Ultrasensitive chemiluminescence detection of DNA on a microfluidic paper-based analytical device
作者：Wang, Yanhu; Wang, Shoumei; Ge, Shenguang; Wang, Shaowei; Yan, Mei; Zang, Dejin; Yu, Jinghua
作者机构：[Wang, Yanhu; Wang, Shoumei; Ge, Shenguang; Wang, Shaowei; Yan, Mei; Zang, Dejin; Yu, Jinghua] Univ Jinan, Sch Chem & Chem Engn, Key Lab Chem Sensing 更多
会议名称：2nd International Congress on Advanced Materials (ICAM)
会议日期：MAY 16-19, 2013
来源：MONATSHEFTE FUR CHEMIE
关键词：Microfluidic paper-based chemiluminescence analytical device; DNA;; Chemiluminescence; Gold nanoparticles
摘要：A novel microfluidic paper-based chemiluminescence (CL) analytical device (mu PAD) with high-throughput, rapid, stable, reusable point-of-care (POC) testing and quantitative response for DNA was designed. Sodium periodate was used to modify mu PAD to covalently immobilize capture DNA. The paper device was fabricated by a low-cost, simple, and rapid wax screen-printing method. The developed paper-based CL immunodevice, combined with a typical luminol-H2O2 CL system, showed excellent analytical performance for the detection of DNA. Moreover, gold nanoparticles for signal DNA immobilization were used for signal-on CL assay of target DNA, which greatly improved the sensitivity. This study shows the successful integration of the mu PAD and the CL method to afford an easy-to-use, inexpensive, and portable alternative for POC monitoring.