标题:The Equally Important Role of Adenine Derivatives to That of Pyrimidine Derivatives in Near-0 eV Electron-Induced DNA Lesions
作者:Wang, Shoushan; Zhao, Peiwen; Zhang, Changzhe; Bu, Yuxiang
作者机构:[Wang, Shoushan; Zhao, Peiwen; Zhang, Changzhe; Bu, Yuxiang] Shandong Univ, Sch Chem & Chem Engn, Inst Theoret Chem, Jinan 250100, Peoples R China.
通讯作者:Bu, YX
通讯作者地址:[Bu, YX]Shandong Univ, Sch Chem & Chem Engn, Inst Theoret Chem, Jinan 250100, Peoples R China.
来源:CHEMPHYSCHEM
出版年:2016
卷:17
期:11
页码:1669-1677
DOI:10.1002/cphc.201600002
关键词:density functional calculations; DNA damage; electron attachment;; nucleobases; proton transfer
摘要:The role of adenine (A) derivatives in DNA damage is scarcely studied due to the low electron affinity of base A. Experimental studies demonstrate that low-energy electron (LEE) attachment to adenine derivatives complexed with amino acids induces barrier-free proton transfer producing the neutral N-7-hydrogenated adenine radicals rather than conventional anionic species. To explore possible DNA lesions at the A sites under physiological conditions, probable bond ruptures in two models-N-7-hydrogenated 2'-deoxyadenosine-3'-monophosphate (3'-dA(N7H)MPH) and 2'-deoxyadenosine-5'-monophosphate (5'-dA(N7H) MPH), without and with LEE attachment-are studied by DFT. In the neutral cases, DNA backbone breakage and base release resulting from C-3'-O-3' and N-9-C-1' bond ruptures, respectively, by an intramolecular hydrogen-transfer mechanism are impossible due to the ultrahigh activation energies. On LEE attachment, the respective C-3'-O-3' and N-9-C-1' bond ruptures in [3'-dA(N7H)MPH](-) and [5'-dA(N7H)MPH](-) anions via a pathway of intramolecular proton transfer (PT) from the C-2' site of 2'-deoxyribose to the C-8 atom of the base moiety become effective, and this indicates that substantial DNA backbone breaks and base release can occur at non-3'-end A sites and the 3'-end A site of a single-stranded DNA in the physiological environment, respectively. In particular, compared to the results of previous theoretical studies, not only are the electron affinities of 3'-dA(N7H) MPH and 5'-dA(N7H) MPH comparable to those of hydrogenated pyrimidine derivatives, but also the lowest energy requirements for the C-3'-O-3' and N-9-glycosidic bond ruptures in [3'-dA(N7H) MPH](-) and [5'dA(N7H) MPH](-) anions, respectively, are comparable to those for the C-3'-O-3' and N-1-glycosidic bond cleavages in corresponding anionic hydrogenated pyrimidine derivatives. Thus, it can be concluded that the role of adenine derivatives in single-stranded DNA damage is equally important to that of pyrimidine derivatives in an irradiated cellular environment.
收录类别:SCOPUS;SCIE
WOS核心被引频次:1
Scopus被引频次:1
资源类型:期刊论文
原文链接:https://www.scopus.com/inward/record.uri?eid=2-s2.0-84973345279&doi=10.1002%2fcphc.201600002&partnerID=40&md5=f2e64c5d628544b94865dabe1c28ef43
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