标题：Replantation of Cryopreserved Fingers: An "Organ Banking" Breakthrough
作者：Wang, Zengtao; Zhu, Lei; Kou, Wei; Sun, Wenhai; He, Bo; Wang, Chunxia; Shen, Yun; Wang, Yang; Zhu, Zhaowei; Liang, You
作者机构：[Wang, Zengtao] Shandong Univ, Prov Hosp, Dept Hand & Foot Surg, 324 Jing 5 Wei 7 Rd, Jinan 250021, Shandong, Peoples R China.; Shandong Univ, Qilu 更多
通讯作者：Wang, ZT;He, B
通讯作者地址：[Wang, ZT]Shandong Univ, Prov Hosp, Dept Hand & Foot Surg, 324 Jing 5 Wei 7 Rd, Jinan 250021, Shandong, Peoples R China;[He, B]Sun Yat Sen Univ, Dept 更多
来源：PLASTIC AND RECONSTRUCTIVE SURGERY
摘要：Background: Although cryopreservation has been widely used in clinical practice, it remains limited to small or thin bloodless tissues with a simple structure and function. In August of 2002, the authors performed the first successful transplantation of a cryopreserved vascularized rat hind limb. Subsequently, the authors extended this cryopreservation technique to the preservation of human fingers. Methods: In December of 2002 and December of 2003, the authors performed two in situ implantations of cryopreserved amputated fingers for two patients by means of the "two-step" and programmed cryopreservation methods. In case 1, computed tomographic angiography was performed on the affected hand 6 months after surgery. In case 2, pieces of skin were obtained from the thawed amputated finger for pathologic examination before replantation. Results: One finger was cryopreserved for 81 days and the other for 5 days. Both fingers were replanted successfully. Computed tomographic angiography revealed a patent radial proper digital artery in case 1. The pathologic results of case 2 showed satisfactory skin cell morphology. After 15- and 14-year follow-up assessments of the two patients, the replanted fingers achieved satisfactory appearance and function. Conclusions: The authors confirmed the effectiveness of deep cryopreservation for the long-term preservation of human fingers. The current application scope of these cryopreservation techniques includes small limbs with minimal amounts of muscle tissue.