标题：Activated STAT3 Could Reduce Survival in Patients with Esophageal Squamous Cell Carcinoma by Up-regulating VEGF and Cyclin D1 Expression
作者：Zhang, Nan; Zhang, Min; Wang, Zhou; Gao, Wei; Sun, Zhi-Gang
作者机构：[Zhang, Nan] Shandong Univ, Jinan Cent Hosp, Dept Oncol, Jinan 250013, Peoples R China.; [Zhang, Nan; Zhang, Min; Gao, Wei; Sun, Zhi-Gang] Shandong 更多
通讯作者：Sun, ZG;Sun, ZG
通讯作者地址：[Sun, ZG]Shandong First Med Univ, Jinan Cent Hosp, Jinan 250013, Peoples R China;[Sun, ZG]Shandong Univ, Jinan Cent Hosp, Dept Thorac Surg, Jinan 2500 更多
来源：JOURNAL OF CANCER
关键词：STAT3; p-STAT3; Esophageal squamous cell cancer; Western Blot;; Immunohistochemistry
摘要：Signal transduction and activators of transcription factor (STAT) 3 is associated with a poor prognosis in certain types of cancer. The purpose of the present study was to investigate the clinical and prognostic significance of STAT3/p-STAT3 expression in esophageal squamous cell cancer (ESCC) patients. A total of 71 patients were enrolled in the study. STAT3 and p-STAT3 expression were detected by Western Blot and immunohistochemistry assays. Stattic, the STAT3 inhibitor, was used to block the activation of STAT3 in ESCC cell lines Eca-109 and Kyse-30, and the CCK8 assay was performed to detect the effect of Stattic on the viability of ESCC cells. The expression of associated genes was assessed by RT-PCR and Western blot at RNA and protein levels, respectively. STAT3 expression was correlated with infiltration degree (pT) and pTNM. And p-STAT3 expression was correlated with pT, lymphatic metastasis (pN) and pTNM. The expression of VEGF, Bcl-xl and Cyclin D1 was up-regulated in ESCC tissues and positively correlated with p-STAT3 level, besides Bcl-xl. In vitro, Stattic inhibited the viability of Eca-109 and Kyse-30 cells in a dose- and time- dependent manner, and significantly inhibited the expression of VEGF, Bcl-xl and CyclinD1 at mRNA and protein level. The 5-year survival rate of the 71 patients was significantly associated with pT, pN, pTNM stage, p-STAT3 level, VEGF expression and Cyclin D1 expression. pN and p-STAT3 expression were independent relevant factors. Our results showed that p-STAT3 might serve as an essential biomarker for tumor invasion and metastasis in ESCC.