标题：Surface modification of coronary stents for intravascular gene delivery
作者：Liu, Lanxia ;Li, Xiaohong ;Zhang, Chao ;Leng, Xigang ;Song, Cunxian
作者机构：[Liu, Lanxia ;Li, Xiaohong ;Zhang, Chao ;Leng, Xigang ;Song, Cunxian ] The Institute of Biomedical Engineering, Chinese Academy of Medical Sciences, T 更多
会议名称：2nd International Conference on Materials Science and Engineering, ICMSE 2013
会议日期：March 8, 2013 - March 10, 2013
来源：Advanced Materials Research
摘要：The present study investigated a novel surface modification on metal coronary stent for antibody immobilization. Methods: 316L stainless steel stents were surface modified with protein coatings. An Anti-DNA antibody was covalently bound to the protein surface using a bi-functional cross-linking agent SPDP. The artwork and binding stability of protein coatings were evaluated by in-vitro eluting. The feasibility and stability of anti-DNA antibody covalently bound to the stent were evaluated by means of 125I labeling. Results: We observed that pre-treating the steel surface using diluted HCL and increasing the ratio of cross-linking agent caused significantly increased binding stability of the protein coatings (p<0.001). The amount of chemically coupled antibody on the stents was 8 times higher than that of physically absorbed control stents. The stability of chemically coupled antibody on the stent was significantly better than physically absorbed control. Conclusion: It is concluded that we optimized the technique of protein coating on stainless steel and achieved stable anti-DNA antibody immobilization, therefore enabled efficient and highly localized non-viral gene delivery. © (2013) Trans Tech Publications, Switzerland.