标题:Sohlh2 inhibits the apoptosis of mouse primordial follicle oocytes via C-kit/PI3K/Akt/Foxo3a signalling pathway
作者:Zhang, Xiaoli;Zhang, Haiyu;Gao, Qing;Ji, Shufang;Bing, Lujun;Hao, Jing
作者机构:[Zhang, X] Department of Histology and Embryology, School of Medicine, Shandong University, Jinan, China;[ Zhang, H] Department of Histology and Embry 更多
通讯作者:Hao, J
通讯作者地址:[Hao, J]Shandong Univ, Dept Histol & Embryol, Sch Med, Key Lab,Minist Educ Expt Teratol, Jinan 250100, Peoples R China.
来源:Reproductive biomedicine online
出版年:2015
卷:30
期:5
页码:514-521
DOI:10.1016/j.rbmo.2015.01.015
关键词:apoptosis;C-kit/PI3K/Akt/Foxo3a;mouse;oocyte;Sohlh2;TUNEL
摘要:We previously reported that bone morphogenetic protein 4/drosophila mothers against decapentaplegic protein (BMP4/Smad) signalling pathway initiated primordial follicle growth and prevented oocyte apoptosis via up-regulation of Sohlh2 and receptor for kit ligand (c-kit). The mechanism underlying this process was not fully elucidated. In the present study, primary oocyte cultures were established from ovaries of 3-day-old female mouse pups by two-step enzyme digestion. Cultures were divided into Sohlh2 small interference RNA (SiRNA) group, negative SiRNA group, Sohlh2 overexpression plasmid group and pCAG-puro group. TdT (terminal deoxynucleotidyl transferase)-mediated dUDP nick-end labelling assay was carried out to detect the oocyte apoptosis; immunocytochemical staining and quantitative real time-polymerase chain reaction detected the expression of c-kit and Forkhead box O3a (Foxo3a); Western blot was performed to detect the expression of Sohlh2, C-kit, saerine/threonine kinases (Akt1) and Foxo3a. The results showed that Sohlh2 inhibited oocyte apoptosis and upregulated c-kit expression; Sohlh2 decreased the endonuclear Foxo3a via the upregulation of phosphorylated Akt1 (P-Akt1) and phosphorylated Foxo3a (P-Foxo3a) but not total Akt1 (T-Akt1) or total Foxo3a (T-Foxo3a); Sohlh2 increased P-Akt1 but not T-Akt1; the PI3K (phosphotidylinsitol-3-kinase) inhibitor LY294002 ameliorated the role of Sohlh2 on phosphorylation of Akt1 and Foxo3a. Sohlh2 may inhibit oocyte apoptosis via c-kit/PI3K/Akt/Foxo3a signalling pathway. (C) 2015 Reproductive Healthcare Ltd. Published by Elsevier Ltd. All rights reserved.
收录类别:SCOPUS;SCIE
WOS核心被引频次:4
Scopus被引频次:5
资源类型:期刊论文
原文链接:https://www.scopus.com/inward/record.uri?eid=2-s2.0-84928927643&doi=10.1016%2fj.rbmo.2015.01.015&partnerID=40&md5=d753051029d26722a500c0a934920cab
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