标题：Oxytocin evokes a pulsatile PGE2 release from ileum mucosa and is required for repair of intestinal epithelium after injury
作者：Chen, Dawei; Zhao, Junhan; Wang, Haoyi; An, Ning; Zhou, Yuping; Fan, Jiahui; Luo, Junwen; Su, Wenlong; Liu, Chuanyong; Li, Jingxin
作者机构：[Chen, Dawei; Zhao, Junhan; Wang, Haoyi; An, Ning; Zhou, Yuping; Fan, Jiahui; Luo, Junwen; Su, Wenlong; Liu, Chuanyong; Li, Jingxin] Shandong Univ, Sc 更多
通讯作者地址：[Li, JX]Shandong Univ, Sch Med, Dept Physiol, Jinan 250012, Peoples R China.
摘要：We measured the short-circuit current (I-sc) in rat ileum mucosa to identify the effect of oxytocin (OT) on mucosal secretion in small intestine. We identified a COX-2-derived pulsatile PGE(2) release triggered by OT in rat ileum mucosa. OT receptors (OTR) are expressed in intestine crypt epithelial cells. Notably, OT evoked a dynamic change of [Ca2+](i) in ileum crypts, which was responsible for this pulsatile release of PGE(2). OT ameliorated (5)-FU-, radiation-or DSS-induced injury in vivo, including the improvement of weight loss, reduced villus height and impaired survival of crypt transitamplifying cells as well as crypt. Moreover, these protective effects of OT against intestinal injury were eliminated by coadministration of a selective inhibitor of PGE(2), AH6809. Our findings strongly suggest that OT, a novel and important regulator of intestine mucosa barrier, is required for repair of intestinal epithelium after injury. Considering that OT is an FDA-approved drug, this work reveals a potential novel and safe way to combat or prevent chemo-radiotherapy induced intestine injury or to treat IBD.