标题：Lower range of molecular weight of xanthan gum inhibits apoptosis of chondrocytes through MAPK signaling pathways
作者：Zhang, Wei; Wu, Jixu; Zhang, Fangfang; Dou, Xixi; Ma, Aibin; Zhang, Xiaogang; Shao, Huarong; Zhao, Shuo; Ling, Peixue; Liu, Fei; H 更多 作者机构：[Zhang, Wei; Han, Guanying] Jinzhou Med Univ, Jinzhou 121001, Peoples R China.; [Zhang, Wei; Zhao, Shuo; Han, Guanying] Jinzhou Med Univ, Affiliated 更多
通讯作者：Han, GY;Liu, F
通讯作者地址：[Han, GY]Jinzhou Med Univ, Affiliated Hosp 1, Jinzhou 121001, Liaoning, Peoples R China;[Liu, F]Shandong Acad Pharmaceut Sci, Shandong Prov Key Lab Bi 更多
来源：INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
关键词：Lower range of molecular weight of xanthan; gum; Apoptosis;; Mitogen-activated protein kinase
摘要：LRWXG has previously been reported to have a protective effect on chondrocytes, preventing apoptosis induced by oxidative stress. In this study, we were aimed at determining whether LRWXG exerts its anti-apoptotic activity through the MAPK signaling pathways in chondrocytes. Our results show that, at the cellular level, apoptosis of chondrocytes in the groups treated by LRWXG decreases compared with groups treated by inhibitors alone and model group under conditions of oxidative stress in a dose-dependent manner. Mechanistically at the molecular level, LRWXG regulates the MAPK pathway induced by oxidative stress: The levels of phosphorylation of JNK and p38 proteins in the groups treated by LRWXG are lower than model group, while compared with corresponding groups of inhibitors, there are no significant difference; For other related proteins, LRWXG reduces the levels of the apoptosis-related proteins BAX and cleaved caspase-3, and increases the level of anti-apoptotic protein BCL2. In addition, LRWXG can significantly reduce the levels of inflammatory-related factors such as COX2, PEG2, TNFa and 1L1 beta, and inhibits the expression of MMPs, increasing the content of type II collagen. The results of this research strongly suggest that LRWXG exerts its anti-apoptotic activity via regulating the MAPK signaling pathways in vitro. (C) 2019 Published by Elsevier B.V.