标题：Smoc2 potentiates proliferation of hepatocellular carcinoma cells via promotion of cell cycle progression
作者：Jing-Ran Su;Jing-Hua Kuai;Yan-Qing Li
作者机构：[Jing-Ran Su;Jing-Hua Kuai;Yan-Qing Li]Department of Gastroenterology, Laboratory of Translational Gastroenterology, Shandong University, Qilu Hospita 更多
通讯作者地址：[Li, YQ]Shandong Univ, Qilu Hosp, Dept Gastroenterol, Lab Translat Gastroenterol, 107 Wenhuaxi Rd, Jinan 250012, Shandong, Peoples R China.
摘要：AIM To determine the influence of Smoc2 on hepatocellular carcinoma(HCC) cell proliferation and to find a possible new therapeutic target for preventing HCC progression.METHODS We detected expression of Smoc2 in HCC tissues and corresponding non-tumor liver(CNL) tissues using PCR, western blot, and immunohistochemistry methods. Subsequently, we down-regulated and upregulated Smoc2 expression using siR NA and lentivirus transfection assay, respectively. Then, we identified the effect of Smoc2 on cell proliferation and cell cycle using CCK-8 and flow cytometry, respectively. The common cell growth signaling influenced by Smoc2 was detected by western blot assay. RESULTS The expression of Smoc2 was significantly higher in HCC tissues compared with CNL tissues. Overexpression of Smoc2 promoted HCC cell proliferation and cell cycle progression. Down-regulation of Smoc2 led to inhibition of cell proliferation and cell cycle progression. Smoc2 had positive effect on ERK and AKT signaling.CONCLUSION Smoc2 promotes the proliferation of HCC cells through accelerating cell cycle progression and might act as an anti-cancer therapeutic target in the future.