标题：Grape seed proanthocyanidins extracts promote apolipoprotein A-I mRNA expression in HepG2 cells under experimental sugar and high-sugar conditions
作者：Zhai, Q.; Zhong, N.; Gao, H. Q.; Li, B. Y.; Jiang, B.
作者机构：[Gao, H. Q.; Li, B. Y.] Shandong Univ, Qilu Hosp, Dept Geriatr, Jinan 250012, Peoples R China.; [Zhai, Q.] Shandong Univ, Qilu Hosp, Dept Intens Car 更多
通讯作者地址：[Gao, HQ]Shandong Univ, Qilu Hosp, Dept Geriatr, Jinan 250012, Peoples R China.
来源：EUROPEAN REVIEW FOR MEDICAL AND PHARMACOLOGICAL SCIENCES
关键词：Grape seed proanthocyanidins extracts; Apolipoprotein A-I; HepG2 cell;; mRNA
摘要：Objectives: In this study, we investigated the effect of grape seed proanthocyanidins extracts (GSPE), which have been proved to have anti-oxidative and anti-aging functions, on the expression of apoA-I at mRNA level of HepG2 cells in vitro under the experimental conditions of high-sugar and sugar.; Materials and Methods: Cell viability was measured by sulforhodamine B (SRB). The apoA-I mRNA expression was assayed by real-time fluorescence quantitative polymerase chain reaction. Firstly, HepG2 cells were incubated in 10% inactivated newborn calf serum in Dulbecco's Modified Eagle Medium (DMEM). Next, cells were incubated with high-sugar and sugar serum-free medium, and added different concentration of GSPE (2.5, 5 and 10 mu g/ml) for more than 24 hours, and thereafter, investigated whether GSPE can promote more apoA-I expression in HepG2 cells under the experimental conditions of high-sugar and sugar.; Results: In this experiment, HepG2 cells were incubated with high-sugar and sugar serum-free medium, and HepG2 cells incubated with high-sugar medium produced less apoA-I at mRNA level. The difference was significant (p < 0.05). When HepG2 cells were incubated with GSPE at concentration of 20 mu g/ml or above for about 4 hours, cell viability measured by SRB was lower than 50%. However, cell viability of HepG2 cells incubated with GSPE at concentration of 10 mu g/ml or below was higher than 70%. Therefore, we chose the HepG2 cells incubated with GSPE concentration of 2.5, 5, 10 mu g/ml to observe the effect of GSPE on the mRNA expression of apoA-I. After incubated with GSPE, the apoA-I expression of HepG2 cells were significantly elevated at mRNA level compared to that of high sugar control (p < 0.05). Moreover, this action of GSPE showed dose dependent, and the dose 2.5 mu g/ml was optimal.; Conclusions: GSPE (concentration of higher than 20 mu g/ml) could inhibit HepG2 cell survival, and in HepG2 cells, endogenous apoA-I was significantly suppressed following 24h of exposure to high concentrations of glucose. Meanwhile GSPE could promote expression of apoA-I dose dependently at mRNA level when its concentration was lower than 10 mu g/ml.